Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 1469337..1470073 Replicon chromosome
Accession NZ_CP081865
Organism Klebsiella pneumoniae strain K5880C

Toxin (Protein)


Gene name tacT Uniprot ID A0A4S4Y1H2
Locus tag K6J95_RS07260 Protein ID WP_032433360.1
Coordinates 1469591..1470073 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag K6J95_RS07255 Protein ID WP_003026799.1
Coordinates 1469337..1469603 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K6J95_RS07230 (1464983) 1464983..1466122 + 1140 WP_032433368.1 mannitol dehydrogenase -
K6J95_RS07235 (1466151) 1466151..1466813 + 663 WP_032433366.1 dihydroxyacetone kinase subunit DhaL -
K6J95_RS07240 (1466797) 1466797..1467801 + 1005 WP_032433364.1 dihydroxyacetone kinase subunit DhaK -
K6J95_RS07245 (1467819) 1467819..1468451 + 633 WP_032433362.1 3-hexulose-6-phosphate synthase -
K6J95_RS07250 (1468461) 1468461..1469024 + 564 WP_032433361.1 6-phospho-3-hexuloisomerase -
K6J95_RS07255 (1469337) 1469337..1469603 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
K6J95_RS07260 (1469591) 1469591..1470073 + 483 WP_032433360.1 GNAT family N-acetyltransferase Toxin
K6J95_RS07265 (1470273) 1470273..1471676 + 1404 WP_210781595.1 ISNCY-like element ISKpn21 family transposase -
K6J95_RS07270 (1471705) 1471705..1472337 - 633 WP_001567369.1 hypothetical protein -
K6J95_RS07275 (1472717) 1472717..1473316 - 600 WP_064472304.1 helix-turn-helix transcriptional regulator -
K6J95_RS07280 (1473529) 1473529..1474473 - 945 WP_077254249.1 fimbrial protein -
K6J95_RS07285 (1474485) 1474485..1475063 - 579 WP_032432061.1 type 1 fimbrial protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1437282..1483299 46017
- flank IS/Tn - - 1470273..1471676 1403


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17421.02 Da        Isoelectric Point: 9.6047

>T213578 WP_032433360.1 NZ_CP081865:1469591-1470073 [Klebsiella pneumoniae]
VGRVTAPEPLSSSHQLAEFVSGETVLDEWLKHRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTETTGRLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYTHHGFTASQTHERTLFLKLP

Download         Length: 483 bp

>T213578 NZ_CP109692:4392442-4392795 [Enterobacter asburiae]
GTGTGGGCAATTAACACGACGGACAGGTTTGATCGCTGGTTCACTTCGCTTGACGATACCGATCGCGCCAGCGTACTTGC
AGCACTGCTTGTTTTGCGAGAGAAGGGGCCGGGTTTATCGAGACCCTACGCTGATACGCTTAAAGGCTCTCGTCACAGCA
ACATGAAAGAGCTACGTATTCAGAGTAAGGGCGATCCCTTGCGGGCGTTTTTCGCCTTTGATCCAAACCGTACTGGCATC
ATGCTTTGTGCTGGCAATAAGGTCGGCAATGAAAGGCGATTCTACGATGAGATGCTTCTCGTTGCTGACCAGGAATTCAC
TCACTGGCTGGATAGATTAAAAGAGAGGGAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT213578 WP_003026799.1 NZ_CP081865:1469337-1469603 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT213578 NZ_CP109692:4392800-4393102 [Enterobacter asburiae]
ATGGGAAGAACCCTTGAGCAGCTTATAGCGGATGAAAAGCCGGAGGTTGTCGCCAGCGCTCAGGCGATAGCAACGGATAT
TTTGCTCAACATTCATCTTGCTGAGTTACGTGAGAAAGTACAAAAAACGCAGGTAGAAATGGCGCAGGCGTTGGGTATCA
GGCAACCTACCGTTGCGGTAATGGAAAAGCCCGGGCGCGATTTGAAACTCTCTTCTCTCAAGCGCTACGTTGAGGCGGCG
GGCGGAAAGCTGCGGCTTGATGTGGAGCTGCCGGACGGCTCGCACTATGAGTTTGTGCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4S4Y1H2

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References