Detailed information of TA system TA021357

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-YefM
• Location: 5288..5874
• Replicon: plasmid 1
• Accession: NC_007615
• Organism: Nitrosospira multiformis ATCC 25196

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q2Y599
• Locus tag: NMUL_RS14510
• Protein ID: WP_011382057.1
• Coordinates: 5288..5632 (-)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q2Y598
• Locus tag: NMUL_RS14515
• Protein ID: WP_011382058.1
• Coordinates: 5629..5874 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NMUL_RS14480	850..1188	+	339	WP_011382052.1	plasmid mobilization relaxosome protein MobC	-
NMUL_RS15090	1229..2368	+	1140	WP_167535604.1	relaxase/mobilization nuclease domain-containing protein	-
NMUL_RS14490	2371..2787	+	417	WP_011382054.1	hypothetical protein	-
NMUL_RS14495	2821..3054	+	234	WP_146063223.1	hypothetical protein	-
NMUL_RS14500	3066..4604	+	1539	WP_011382055.1	TraM recognition domain-containing protein	-
NMUL_RS14505	4601..5188	+	588	WP_011382056.1	hypothetical protein	-
NMUL_RS14510	5288..5632	-	345	WP_011382057.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
NMUL_RS14515	5629..5874	-	246	WP_011382058.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
NMUL_RS14520	6171..6557	+	387	WP_011382059.1	type II toxin-antitoxin system RelE/ParE family toxin	-
NMUL_RS14525	6554..6907	+	354	WP_011382060.1	helix-turn-helix domain-containing protein	-
NMUL_RS14530	7010..7381	+	372	WP_011382061.1	XRE family transcriptional regulator	-
NMUL_RS14535	7404..8432	-	1029	WP_104009784.1	chromosomal replication initiator DnaA	-
NMUL_RS14540	9045..10016	+	972	WP_011382063.1	tyrosine-type recombinase/integrase	-
NMUL_RS15845	10328..10831	-	504	WP_146063224.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..18871	18871

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 13353.21 Da
• Isoelectric Point: 5.7829

>T21357 WP_011382057.1 NC_007615:c5632-5288 [Nitrosospira multiformis ATCC 25196]
MKRYILSPAAKTDITNIRKYTIQKWGKSQADKYTLELRERMRWLADNPMLGRARDEVKEGYRSFKEGSHIIFYRVVESTI
EIIGIPHQNMDIEQNLEEEILLFTNIADGGFEGD

Nucleotide

• Length: 345 bp

>T21357 NC_007615:c5632-5288 [Nitrosospira multiformis ATCC 25196]
ATGAAACGTTACATCCTCTCGCCGGCCGCAAAAACCGATATCACCAACATACGTAAATACACGATCCAGAAATGGGGAAA
ATCCCAAGCCGACAAGTACACCCTGGAACTTCGCGAACGGATGCGCTGGCTGGCAGACAACCCCATGCTGGGACGGGCAA
GAGATGAAGTAAAGGAAGGCTACCGCAGTTTCAAGGAAGGCAGCCACATAATATTTTACCGGGTAGTTGAAAGCACAATA
GAGATTATTGGCATTCCGCACCAGAATATGGATATTGAACAGAATCTGGAAGAGGAAATTCTCCTTTTCACAAATATTGC
GGATGGTGGGTTTGAAGGGGATTGA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8872.17 Da
• Isoelectric Point: 5.1442

>AT21357 WP_011382058.1 NC_007615:c5874-5629 [Nitrosospira multiformis ATCC 25196]
MKAIAAKEAKNNFGEMLDTVQREPLTIEKHGRAVAVVMSAREYQQMKLERLQAKLAVGEGQLNRGEGVDGEAVFAELLSE
A

Nucleotide

• Length: 246 bp

>AT21357 NC_007615:c5874-5629 [Nitrosospira multiformis ATCC 25196]
ATGAAAGCTATTGCTGCCAAAGAGGCAAAAAATAATTTTGGAGAAATGCTGGACACCGTTCAGCGGGAACCACTTACCAT
TGAAAAACATGGGCGAGCTGTTGCCGTGGTTATGTCAGCCCGGGAATACCAACAGATGAAACTTGAAAGGTTGCAGGCGA
AACTGGCTGTAGGTGAGGGACAGCTAAACAGGGGGGAAGGGGTGGACGGCGAAGCAGTTTTTGCCGAACTGTTAAGTGAA
GCATGA

Structures

Toxin

• AlphaFold DB: Q2Y599

Antitoxin

• AlphaFold DB: Q2Y598