Detailed information of TA system TA021355

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): virT-dinJ/YafQ-RelB
• Location: 2329094..2329637
• Replicon: chromosome
• Accession: NC_007614
• Organism: Nitrosospira multiformis ATCC 25196

Toxin (Protein)

• Gene name: virT
• Uniprot ID: Q2Y7E2
• Locus tag: NMUL_RS10645
• Protein ID: WP_011381344.1
• Coordinates: 2329356..2329637 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: Q2Y7E3
• Locus tag: NMUL_RS10640
• Protein ID: WP_011381343.1
• Coordinates: 2329094..2329369 (+)
• Length: 92 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NMUL_RS16195	2324669..2324845	-	177	WP_202944824.1	hypothetical protein	-
NMUL_RS10620	2325158..2325697	-	540	WP_011381341.1	FxDxF family PEP-CTERM protein	-
NMUL_RS10630	2326874..2327134	+	261	WP_041352558.1	hypothetical protein	-
NMUL_RS10635	2327271..2328368	+	1098	WP_011381342.1	sulfate ABC transporter substrate-binding protein	-
NMUL_RS15770	2328624..2328861	-	238	Protein_2045	S26 family signal peptidase	-
NMUL_RS10640	2329094..2329369	+	276	WP_011381343.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
NMUL_RS10645	2329356..2329637	+	282	WP_011381344.1	type II toxin-antitoxin system YafQ family toxin	Toxin
NMUL_RS10650	2330518..2331285	+	768	WP_011381345.1	Crp/Fnr family transcriptional regulator	-
NMUL_RS10655	2331471..2331938	+	468	WP_011381346.1	response regulator	-
NMUL_RS10660	2332762..2333469	-	708	WP_104009778.1	PEP-CTERM sorting domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-88)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10742.60 Da
• Isoelectric Point: 10.4110

>T21355 WP_011381344.1 NC_007614:2329356-2329637 [Nitrosospira multiformis ATCC 25196]
MRRIKFSHTFKRDFRRVKSQSPHRDIDSLLKTTMDLLVTDMPLPASCVDHALKGKLKGYRDCHIKPDVVLIYRKQGNDVL
ELVRLGSHSELGI

Nucleotide

• Length: 282 bp

>T21355 NC_007614:2329356-2329637 [Nitrosospira multiformis ATCC 25196]
ATGCGCCGGATTAAGTTTTCGCACACCTTCAAGCGCGATTTCCGGCGCGTGAAGTCTCAGTCACCACATCGGGACATTGA
CTCTCTGCTGAAAACTACAATGGACTTGTTGGTAACTGATATGCCTTTGCCAGCAAGCTGCGTTGACCACGCCTTGAAGG
GAAAATTGAAAGGGTATCGGGATTGCCACATTAAACCAGATGTGGTGTTGATTTATCGCAAGCAAGGCAATGACGTTCTC
GAACTCGTACGCCTCGGCTCACATAGCGAACTTGGCATTTAG

Antitoxin

Protein

• Length: 92 a.a.
• Molecular weight: 10257.65 Da
• Isoelectric Point: 4.1571

>AT21355 WP_011381343.1 NC_007614:2329094-2329369 [Nitrosospira multiformis ATCC 25196]
MSKTDIVRARIDAQVKEEASRVLGEMGLTPSDGIRMFLTSVAKKKELPFHPMDFFEPNAETIAAMEEVEREENITACETV
EELMEQLNAPD

Nucleotide

• Length: 276 bp

>AT21355 NC_007614:2329094-2329369 [Nitrosospira multiformis ATCC 25196]
ATGTCCAAAACCGACATCGTGCGCGCCCGAATTGACGCACAAGTGAAAGAAGAAGCAAGCCGAGTATTGGGAGAAATGGG
CCTGACACCCTCAGACGGCATACGCATGTTTTTAACAAGTGTGGCCAAGAAAAAAGAGTTGCCGTTTCACCCCATGGATT
TCTTCGAACCGAATGCCGAAACCATTGCCGCCATGGAAGAAGTAGAACGCGAGGAAAATATAACGGCGTGTGAAACAGTC
GAAGAATTAATGGAACAATTGAATGCGCCGGATTAA

Structures

Toxin

• AlphaFold DB: Q2Y7E2

Antitoxin

• AlphaFold DB: Q2Y7E3