Detailed information of TA system TA213546

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 787653..788299
• Replicon: plasmid p29362-2
• Accession: NZ_CP081849
• Organism: Polymorphobacter megasporae strain PAMC 29362

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KTC28_RS21815
• Protein ID: WP_216711222.1
• Coordinates: 787653..788042 (-)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: KTC28_RS21820
• Protein ID: WP_216711223.1
• Coordinates: 788042..788299 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KTC28_RS21795 (KTC28_21795)	782823..783113	-	291	WP_216711218.1	helix-turn-helix transcriptional regulator	-
KTC28_RS21800 (KTC28_21800)	783130..784389	-	1260	WP_216711219.1	tyrosine-type recombinase/integrase	-
KTC28_RS21805 (KTC28_21805)	784386..785765	-	1380	WP_216711220.1	site-specific integrase	-
KTC28_RS21810 (KTC28_21810)	786791..787336	-	546	WP_216711221.1	hypothetical protein	-
KTC28_RS21815 (KTC28_21815)	787653..788042	-	390	WP_216711222.1	type II toxin-antitoxin system VapC family toxin	Toxin
KTC28_RS21820 (KTC28_21820)	788042..788299	-	258	WP_216711223.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KTC28_RS21825 (KTC28_21825)	788955..789884	-	930	WP_216711224.1	LysR family transcriptional regulator	-
KTC28_RS21830 (KTC28_21830)	790002..790700	+	699	WP_216711225.1	pirin family protein	-
KTC28_RS21835 (KTC28_21835)	790899..791315	+	417	WP_216711226.1	DoxX family protein	-
KTC28_RS21840 (KTC28_21840)	791418..791858	-	441	WP_216711227.1	nuclear transport factor 2 family protein	-
KTC28_RS21845 (KTC28_21845)	791951..792517	-	567	WP_255602654.1	cytochrome c	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..906785	906785

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-124)

Antitoxin

• Antitoxin VapB-like (1-84)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 13895.84 Da
• Isoelectric Point: 6.2305

>T213546 WP_216711222.1 NZ_CP081849:c788042-787653 [Polymorphobacter megasporae]
MVIDSSALMAILRREPEEQDFRNAIKLAASRLISASTRVEVGIVALGRAGEAGLEQLQALLDTLRVETVPVSADHASLAI
DAFRRFGKGRHPAGLNFGDCFSYALAKATGEPLLFKGQDFSHTDIERAA

Nucleotide

• Length: 390 bp

>T213546 NZ_CP109614:c5153484-5153101 [Klebsiella sp. KP20-425-1]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9583.72 Da
• Isoelectric Point: 4.5393

>AT213546 WP_216711223.1 NZ_CP081849:c788299-788042 [Polymorphobacter megasporae]
MALSLKDRETDSLARQVASLTGETLTEAVRGALRLRLRDEQLKRGERPWDDAAIDAIIERFNALPLLDHRTDDDILGYDE
HGLPS

Nucleotide

• Length: 258 bp

>AT213546 NZ_CP109614:c5153726-5153484 [Klebsiella sp. KP20-425-1]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA