Detailed information of TA system TA213543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 14211..14794
• Replicon: plasmid p1
• Accession: NZ_CP081845
• Organism: Caenibius sp. WL

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: K5X80_RS16835
• Protein ID: WP_222558346.1
• Coordinates: 14540..14794 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K5X80_RS16830
• Protein ID: WP_222558347.1
• Coordinates: 14211..14543 (-)
• Length: 111 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5X80_RS16815 (K5X80_16805)	9511..10485	+	975	WP_222558350.1	hypothetical protein	-
K5X80_RS16820 (K5X80_16810)	10517..11281	+	765	WP_222558349.1	hypothetical protein	-
K5X80_RS16825 (K5X80_16815)	11560..14100	+	2541	WP_222558348.1	hypothetical protein	-
K5X80_RS16830 (K5X80_16820)	14211..14543	-	333	WP_222558347.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
K5X80_RS16835 (K5X80_16825)	14540..14794	-	255	WP_222558346.1	type II toxin-antitoxin system HicA family toxin	Toxin
K5X80_RS16840 (K5X80_16830)	14885..16171	+	1287	WP_222558345.1	hypothetical protein	-
K5X80_RS16845 (K5X80_16835)	16153..16443	+	291	WP_222558344.1	hypothetical protein	-
K5X80_RS16850 (K5X80_16840)	16445..16996	+	552	WP_222558343.1	glycosyl hydrolase 108 family protein	-
K5X80_RS16855 (K5X80_16845)	16993..17361	+	369	WP_222558342.1	hypothetical protein	-
K5X80_RS16860 (K5X80_16850)	17429..17803	+	375	WP_222558341.1	hypothetical protein	-
K5X80_RS16865 (K5X80_16855)	18176..18604	+	429	WP_222558340.1	hypothetical protein	-
K5X80_RS16870 (K5X80_16860)	18590..19183	-	594	WP_222558339.1	SOS response-associated peptidase family protein	-
K5X80_RS16875 (K5X80_16865)	19234..19557	+	324	WP_222558338.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38127	38127

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-77)

Antitoxin

• Uncharacterised protein family HicB (54-104)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9339.79 Da
• Isoelectric Point: 9.9740

>T213543 WP_222558346.1 NZ_CP081845:c14794-14540 [Caenibius sp. WL]
MNSKQSKTLKAIFSDPVSGTIKWASIESLFVAVGCEIIEGKGSHVQAHKDGVIGYFVRPHPNKEAKRYQIRDVREFLAKI
GVEP

Nucleotide

• Length: 255 bp

>T213543 NZ_CP109614:c4559724-4559191 [Klebsiella sp. KP20-425-1]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 111 a.a.
• Molecular weight: 12245.75 Da
• Isoelectric Point: 5.1687

>AT213543 WP_222558347.1 NZ_CP081845:c14543-14211 [Caenibius sp. WL]
MNVMSHKGYSARIEYDDDDGIFFGRLAGINDVVGFHADSVEELRAAFHEAVDDYIEACAKIGKDPQKPYSGKMMFRVSPE
LHRRAAIAAELAGKSLNQWAEEALHEALPH

Nucleotide

• Length: 333 bp

>AT213543 NZ_CP109614:c4560001-4559735 [Klebsiella sp. KP20-425-1]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA