Detailed information of TA system TA213541

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE(toxin)
• Location: 6572..7065
• Replicon: plasmid pBN31-16k
• Accession: NZ_CP081835
• Organism: Vagococcus lutrae strain BN31

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V6Q3V2
• Locus tag: K5X77_RS10115
• Protein ID: WP_023606340.1
• Coordinates: 6572..6847 (-)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V6Q580
• Locus tag: K5X77_RS10120
• Protein ID: WP_023606341.1
• Coordinates: 6844..7065 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5X77_RS10075 (K5X77_10075)	1726..2115	-	390	WP_222319392.1	hypothetical protein	-
K5X77_RS10080 (K5X77_10080)	2126..2620	-	495	WP_222319394.1	hypothetical protein	-
K5X77_RS10085 (K5X77_10085)	2728..3672	-	945	WP_222319396.1	DnaA/Hda family protein	-
K5X77_RS10090 (K5X77_10090)	3693..4646	-	954	WP_222319398.1	GntR family transcriptional regulator	-
K5X77_RS10095 (K5X77_10095)	4661..5197	-	537	WP_222319400.1	hypothetical protein	-
K5X77_RS10100 (K5X77_10100)	5319..5486	-	168	WP_222319402.1	hypothetical protein	-
K5X77_RS10105 (K5X77_10105)	5527..5727	-	201	WP_246616582.1	transcriptional regulator	-
K5X77_RS10110 (K5X77_10110)	5905..6534	+	630	WP_222319404.1	helix-turn-helix transcriptional regulator	-
K5X77_RS10115 (K5X77_10115)	6572..6847	-	276	WP_023606340.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K5X77_RS10120 (K5X77_10120)	6844..7065	-	222	WP_023606341.1	DUF6290 family protein	Antitoxin
K5X77_RS10125 (K5X77_10125)	7977..8270	+	294	WP_222319406.1	hypothetical protein	-
K5X77_RS10130 (K5X77_10130)	8658..9578	-	921	WP_222319364.1	Abi family protein	-
K5X77_RS10135 (K5X77_10135)	9606..10142	-	537	WP_222319366.1	hypothetical protein	-
K5X77_RS10140 (K5X77_10140)	10149..10898	-	750	WP_172442040.1	replication initiation protein	-
K5X77_RS10145 (K5X77_10145)	11252..11815	-	564	WP_222319368.1	recombinase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..16159	16159

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (8-90)

Antitoxin

• Protein of unknown function DUF6290 (1-71)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10753.42 Da
• Isoelectric Point: 10.4608

>T213541 WP_023606340.1 NZ_CP081835:c6847-6572 [Vagococcus lutrae]
MTQKKYTVRYEKNAQKALKKMDKHQAGLIMSWISKNLNETEFPRHHGKALAGNKSGQWRYRVGDYRIIAHIDDDTITILV
LEIGHRREIYK

Nucleotide

• Length: 276 bp

>T213541 NZ_CP109614:c2622899-2621577 [Klebsiella sp. KP20-425-1]
ATGGCGACGCTTACCACCTGGATGAACAATGTGCGCGTGGGAACCTTAACCCGCCAGGCTAACGGGGCGCACAGTTTTCG
CTATGACGAAGAGTGGCTGCGCAGCCCGCGCGCCAGACCGCTCTCCCTCTCACTGCCGCTGCAGTATGGCAATATCACTG
CCGACGCGGTGTATCACTATTTTGATAACCTGCTGCCCGACAGCCCGCAGGTCCGCGACCGGATCGTCAGGCGCTATCAG
GCGCGCTCGAAGCAGCCGTTCGATCTGCTGGCCGAAGTCGGGCGGGATAGCGTAGGGGCGGTGACCCTCCTGCCGCCGGG
GGAAGAGGCGCACCTGGAAGGATTACGCTGGCAGACGCTGGACGAGGCGCAGCTCACCGCTTTGCTGACCGCTTACCAGT
CGGATATCCCCCTCGGGATGATTACCAGCCAGGACGATTTCCGTATCTCGGTGGCCGGCGCCCAGGAGAAAACGGCGCTG
CTGCGGATGGGCGAGCAGTGGTGTATTCCGCAGGGCGCCACGCCGACCACGCATATCATTAAGCTGCCCATCGGCGAGAT
CAAGCAGCCCAATGCGACGCTGGATCTGCGTGAAAGCGTGGATAATGAGTATCTGTGCCTGGCGCTGGCCAGAGAACTGG
GCCTGGCGGTGCCGGAAGCGGAGATCATCACCACCCCGCGGATCCGCGCCCTGGCGGTAACCCGCTTCGACCGGCGCTGG
GCGCAGGAGGGACGGGTGCTGCTGCGTCTGCCGCAGGAGGATCTGTGTCAGGCTTTTGGTCTTCCTTCAGCGATGAAATA
TGAGTCTGACGGCGGGCCGGGTATCGCCGCGATCATGACCTTTCTGCTGGGCTCGAGTGAAGCGCTGAAAGATCGCTATG
ACTTTATGAAGTTCATGGTCTTCCAGTGGCTGACGGGGGCGACGGACGGTCACGCAAAAAACTTCTCAATTTACCTGTTG
CCCGGCGGCAGCTACCGCTTAACACCGTTCTACGACATTATTTCGGCGTTTCCGGTGCTGGGCGGCACGGGACTGCATCT
GCGCGATCTGAAGCTGTCGATGGGGCTCAATGCCACAAAAGGGCGTAAAACAGAGATTAACGCCATCTATCCGCGTCATT
TTCTGGCGACGGCCAAAGCGGTGAACTTTCCCCGGGAGCAGATGCTGGCGATCCTCCAGGAGTTTGCCGGGCATGTGCCG
CAGGCAATCGAAAGCGCTCGCCGGACGCTGCCGGCGGACTTTTCCAGCCACGTCTGGCAGGCCATTACAGAGAACATGCT
GAAGCTGCACGCCCGCCTGCAGCAAGGGCTGCAGGCGCTATAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8357.54 Da
• Isoelectric Point: 4.2925

>AT213541 WP_023606341.1 NZ_CP081835:c7065-6844 [Vagococcus lutrae]
MSNVTFRVTDEEKKFMQSMAELNGLSLSELARTKLLEALEDQIDIGLYEKAMKAHELKDESISHEEMLQELGL

Nucleotide

• Length: 222 bp

>AT213541 NZ_CP109614:c2623165-2622899 [Klebsiella sp. KP20-425-1]
ATGAATTCCCCGCAGATATACAGTCCCGTGCAGCTGGCCAATTACCTGAAGCTGCTGCGCCAGCAGAACCACTGGACCCA
GGATGCGCTGGCCAGGCGGATCGGCATTAAGCAGGCCACGCTGTCCAATTTTGAAAATCACCCCGACAAAACCACCTTAA
CCACGCTGTTTAAGATCCTCCAGTCGCTGGGGGTGGTGATGACGCTGAACGCCAAAGCTGCTGAGACGGCGCCGGCTCCC
GATAACAACACGGACCTTGACTGGTAA

Structures

Toxin

• AlphaFold DB: V6Q3V2

Antitoxin

• AlphaFold DB: V6Q580