Detailed information of TA system TA213540

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 14615..15752
• Replicon: plasmid pBN31-cfrD
• Accession: NZ_CP081834
• Organism: Vagococcus lutrae strain BN31

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: K5X77_RS09945
• Protein ID: WP_222319361.1
• Coordinates: 14889..15752 (+)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: F0EPW7
• Locus tag: K5X77_RS09940
• Protein ID: WP_005237730.1
• Coordinates: 14615..14887 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5X77_RS09910 (K5X77_09910)	10319..10999	+	681	WP_044291606.1	IS6-like element IS1216 family transposase	-
K5X77_RS09915 (K5X77_09915)	11033..11344	-	312	WP_172443271.1	helix-turn-helix domain-containing protein	-
K5X77_RS09920 (K5X77_09920)	11789..12862	+	1074	WP_216807377.1	Cfr family 23S rRNA (adenine(2503)-C(8))-methyltransferase	-
K5X77_RS09925 (K5X77_09925)	12946..13680	-	735	Protein_17	glutamine-hydrolyzing GMP synthase	-
K5X77_RS09930 (K5X77_09930)	13743..14423	-	681	WP_044291606.1	IS6-like element IS1216 family transposase	-
K5X77_RS09935 (K5X77_09935)	14494..14598	+	105	Protein_19	peptide-binding protein	-
K5X77_RS09940 (K5X77_09940)	14615..14887	+	273	WP_005237730.1	antitoxin	Antitoxin
K5X77_RS09945 (K5X77_09945)	14889..15752	+	864	WP_222319361.1	zeta toxin family protein	Toxin
K5X77_RS09950 (K5X77_09950)	16193..16510	+	318	WP_002300567.1	hypothetical protein	-
K5X77_RS09955 (K5X77_09955)	17044..17541	+	498	WP_002300565.1	molecular chaperone DnaJ	-
K5X77_RS09960 (K5X77_09960)	17575..17808	+	234	WP_002332654.1	hypothetical protein	-
K5X77_RS09965 (K5X77_09965)	17902..18159	-	258	WP_002325623.1	hypothetical protein	-
K5X77_RS09970 (K5X77_09970)	18162..18461	-	300	WP_002301627.1	hypothetical protein	-
K5X77_RS09975 (K5X77_09975)	18744..20738	+	1995	WP_229256251.1	MobQ family relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / Cfr(D)	-	1..33485	33485
	-	inside	IScluster/Tn	erm(B) / Cfr(D)	-	6623..14429	7806

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (26-213)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32447.95 Da
• Isoelectric Point: 6.3643

>T213540 WP_222319361.1 NZ_CP081834:14889-15752 [Vagococcus lutrae]
MANIVNFTDKQFENRLNDNLEELVQGKKAAESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLEMPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGL

Nucleotide

• Length: 864 bp

>T213540 NZ_CP109614:1910907-1911009 [Klebsiella sp. KP20-425-1]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10735.27 Da
• Isoelectric Point: 6.4687

>AT213540 WP_005237730.1 NZ_CP081834:14615-14887 [Vagococcus lutrae]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFEYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT213540 NZ_CP109614:c1911016-1910871 [Klebsiella sp. KP20-425-1]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Structures

Antitoxin

• AlphaFold DB: A0A475M8I8