Detailed information of TA system TA021354

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-MazE
• Location: 1143423..1144065
• Replicon: chromosome
• Accession: NC_007614
• Organism: Nitrosospira multiformis ATCC 25196

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: Q2YAD4
• Locus tag: NMUL_RS05185
• Protein ID: WP_011380332.1
• Coordinates: 1143423..1143845 (-)
• Length: 141 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q2YAD3
• Locus tag: NMUL_RS05190
• Protein ID: WP_011380333.1
• Coordinates: 1143832..1144065 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NMUL_RS05155	1138820..1139131	-	312	WP_011380326.1	NAD(P)H-dependent oxidoreductase subunit E	-
NMUL_RS05160	1139170..1140270	-	1101	WP_011380327.1	spermidine/putrescine ABC transporter substrate-binding protein	-
NMUL_RS05165	1140294..1141097	-	804	WP_011380328.1	ABC transporter permease subunit	-
NMUL_RS05170	1141099..1141998	-	900	WP_011380329.1	ABC transporter permease	-
NMUL_RS05175	1142010..1143101	-	1092	WP_011380330.1	ABC transporter ATP-binding protein	-
NMUL_RS05180	1143144..1143377	-	234	WP_167535556.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
NMUL_RS05185	1143423..1143845	-	423	WP_011380332.1	PIN domain-containing protein	Toxin
NMUL_RS05190	1143832..1144065	-	234	WP_011380333.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
NMUL_RS15270	1144099..1144485	+	387	WP_202944853.1	recombinase family protein	-
NMUL_RS16115	1144544..1144597	+	54	WP_202944854.1	helix-turn-helix domain-containing protein	-
NMUL_RS05200	1144740..1145816	+	1077	WP_011380335.1	hypothetical protein	-
NMUL_RS05205	1146418..1147932	-	1515	WP_011380336.1	ubiquinone biosynthesis regulatory protein kinase UbiB	-
NMUL_RS05210	1147998..1148579	-	582	WP_011380337.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-115)

Antitoxin

• SpoVT-AbrB domain (5-47)

Sequences

Toxin

Protein

• Length: 141 a.a.
• Molecular weight: 15838.27 Da
• Isoelectric Point: 5.1797

>T21354 WP_011380332.1 NC_007614:c1143845-1143423 [Nitrosospira multiformis ATCC 25196]
MSATSFIYTNILLYLLSPSAAKANRAEEVLRGGGRISVQVLNEMASVMRRKLSMPWHEVNDVLFLIRSICPVEPLTLEVH
DRGRRVAERWGLSLYDGMIAAAALLSNCDILYSEDMQHDLLIDEQLRIQNPFFVNCNGEN

Nucleotide

• Length: 423 bp

>T21354 NC_007614:c1143845-1143423 [Nitrosospira multiformis ATCC 25196]
ATGAGCGCGACTAGCTTTATCTACACAAATATTCTTCTATATCTGCTCTCTCCGAGTGCGGCCAAGGCAAACCGCGCTGA
AGAAGTCCTGCGAGGTGGCGGCAGGATCAGTGTACAGGTCCTTAACGAAATGGCCAGCGTCATGCGCCGCAAGCTTTCCA
TGCCATGGCATGAGGTCAACGATGTGCTTTTCCTGATCCGCTCAATTTGTCCCGTCGAACCGCTGACGCTGGAAGTACAC
GACCGGGGGCGGCGGGTTGCAGAGCGGTGGGGCCTGAGCTTGTACGATGGGATGATTGCAGCGGCGGCGCTGCTCTCAAA
CTGCGATATTCTATATTCTGAGGACATGCAGCACGATTTGCTGATAGACGAGCAATTGCGAATACAGAATCCCTTTTTCG
TAAACTGCAATGGCGAAAACTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 9017.20 Da
• Isoelectric Point: 5.2282

>AT21354 WP_011380333.1 NC_007614:c1144065-1143832 [Nitrosospira multiformis ATCC 25196]
MQVSRWGNSLAIRLPVSVVEALDLREGDDIEVIVAGDRKFEIRKKPGADEFLERLRKYRGRLPADFRFDREEANERD

Nucleotide

• Length: 234 bp

>AT21354 NC_007614:c1144065-1143832 [Nitrosospira multiformis ATCC 25196]
ATGCAAGTTTCAAGATGGGGTAACAGCCTTGCTATCCGCCTGCCGGTTTCAGTAGTGGAAGCTCTTGATCTGCGCGAAGG
CGACGATATTGAAGTAATCGTTGCCGGCGACAGGAAATTCGAGATCAGAAAAAAACCGGGGGCAGATGAATTTCTGGAAA
GGTTGCGCAAGTATCGGGGCAGACTTCCCGCCGATTTCAGGTTTGACCGGGAAGAAGCGAATGAGCGCGACTAG

Structures

Toxin

• AlphaFold DB: Q2YAD4

Antitoxin

• AlphaFold DB: Q2YAD3