Detailed information of TA system TA213539

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 9114..10281
• Replicon: plasmid pBN31-cfrD
• Accession: NZ_CP081834
• Organism: Vagococcus lutrae strain BN31

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: K5X77_RS09905
• Protein ID: WP_222319359.1
• Coordinates: 9388..10281 (+)
• Length: 298 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: B3CKC7
• Locus tag: K5X77_RS09900
• Protein ID: WP_002333002.1
• Coordinates: 9114..9386 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5X77_RS09870 (K5X77_09870)	6127..6366	+	240	WP_002425012.1	peptide-binding protein	-
K5X77_RS09875 (K5X77_09875)	6430..6498	+	69	WP_011100846.1	23S rRNA methyltransferase attenuator leader peptide ErmL	-
K5X77_RS09880 (K5X77_09880)	6623..7360	+	738	WP_032495453.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
K5X77_RS09885 (K5X77_09885)	7530..7790	+	261	Protein_9	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
K5X77_RS09890 (K5X77_09890)	7893..8789	+	897	WP_222319357.1	ParA family protein	-
K5X77_RS09895 (K5X77_09895)	8881..9096	+	216	WP_001835296.1	peptide-binding protein	-
K5X77_RS09900 (K5X77_09900)	9114..9386	+	273	WP_002333002.1	antitoxin	Antitoxin
K5X77_RS09905 (K5X77_09905)	9388..10281	+	894	WP_222319359.1	zeta toxin family protein	Toxin
K5X77_RS09910 (K5X77_09910)	10319..10999	+	681	WP_044291606.1	IS6-like element IS1216 family transposase	-
K5X77_RS09915 (K5X77_09915)	11033..11344	-	312	WP_172443271.1	helix-turn-helix domain-containing protein	-
K5X77_RS09920 (K5X77_09920)	11789..12862	+	1074	WP_216807377.1	Cfr family 23S rRNA (adenine(2503)-C(8))-methyltransferase	-
K5X77_RS09925 (K5X77_09925)	12946..13680	-	735	Protein_17	glutamine-hydrolyzing GMP synthase	-
K5X77_RS09930 (K5X77_09930)	13743..14423	-	681	WP_044291606.1	IS6-like element IS1216 family transposase	-
K5X77_RS09935 (K5X77_09935)	14494..14598	+	105	Protein_19	peptide-binding protein	-
K5X77_RS09940 (K5X77_09940)	14615..14887	+	273	WP_005237730.1	antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / Cfr(D)	-	1..33485	33485
	-	inside	IScluster/Tn	erm(B) / Cfr(D)	-	6623..14429	7806

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 298 a.a.
• Molecular weight: 33516.29 Da
• Isoelectric Point: 8.2780

>T213539 WP_222319359.1 NZ_CP081834:9388-10281 [Vagococcus lutrae]
MANIVNFTDKQFENRLSDNLEELVQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDKGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIAVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKISGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPAIPKTPKLPGVLLQSFKSTIK

Nucleotide

• Length: 894 bp

>T213539 NZ_CP109614:1466980-1467462 [Klebsiella sp. KP20-425-1]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.29 Da
• Isoelectric Point: 7.1987

>AT213539 WP_002333002.1 NZ_CP081834:9114-9386 [Vagococcus lutrae]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELKAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT213539 NZ_CP109614:1466726-1466992 [Klebsiella sp. KP20-425-1]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2Z6BWJ3