Detailed information of TA system TA213537

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 147..621
• Replicon: plasmid pAR8538_2
• Accession: NZ_CP081831
• Organism: Klebsiella quasipneumoniae strain AR8538

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: K5P22_RS27840
• Protein ID: WP_064183949.1
• Coordinates: 147..413 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: K5P22_RS27845
• Protein ID: WP_064183950.1
• Coordinates: 397..621 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5P22_RS27835	1..5101	-	5101	WP_074377680.1	hypothetical protein	-
K5P22_RS27840	147..413	-	267	WP_064183949.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K5P22_RS27845	397..621	-	225	WP_064183950.1	TraY domain-containing protein	Antitoxin
K5P22_RS27850	780..1028	+	249	WP_074384346.1	hypothetical protein	-
K5P22_RS27855	1776..2177	-	402	WP_009310048.1	helix-turn-helix domain-containing protein	-
K5P22_RS27860	2177..2368	-	192	WP_004187666.1	Rop family plasmid primer RNA-binding protein	-
K5P22_RS27865	2786..2936	+	151	Protein_6	replication initiation protein	-
K5P22_RS27870	3183..4052	-	870	WP_064183947.1	WYL domain-containing protein	-
K5P22_RS27875	4680..4910	+	231	WP_074377679.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..5101	5101

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Relaxosome protein TraY (4-45)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10415.06 Da
• Isoelectric Point: 7.9924

>T213537 WP_064183949.1 NZ_CP081831:c413-147 [Klebsiella quasipneumoniae]
MVWTINYSERALKSLRKMDKQTARRILDFMDLRIAVAEDPRQSGKPLKGELGEFWRYRVGDYRILCEILDDELVILAATI
GHRREVYD

Nucleotide

• Length: 267 bp

>T213537 NZ_CP109614:833956-834366 [Klebsiella sp. KP20-425-1]
GTGGTCCTGTGGCAATCTGATCTCCGTATCTCCTGGCGCGCGCAGTGGTTTTCCCTGCTGCTTCACGGCGTGGTGGCGGC
CCTGGTGCTGTTGGTTCCCTGGCCGCTGAGCTATACCCCGATCTGGCTGCTGCTGCTGTCGCTGGTGGTCTTCGACTGCG
TGCGCAGCCAGCGGCGGATCCATGCCCGTCGCGGGGAGATAAAACTGCTCACCGATTCCCGTCTTCGCTGGCAAAACGCC
GAATGGGAGATCCTCGGGACGCCGTGGGTTATCAATAGCGGTATGCTGCTGCGTTTGCGCCATGTCGATACCCGGCGCGG
ACAGCATCTTTGGCTGGCGGCGGACAGCATGGACGCCGGAGAGTGGCGCGATCTGCGCCGGCTGGTGCTGCAAAAACCGG
CGCAGGAGTAA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8307.39 Da
• Isoelectric Point: 5.7065

>AT213537 WP_064183950.1 NZ_CP081831:c621-397 [Klebsiella quasipneumoniae]
MLAIRLSAEIESRLDLLAKKTGRTKTFYAREAILAHLEDLEDYYLSAETAARVRRGDEAVHSSEAVRKSLGLDD

Nucleotide

• Length: 225 bp

>AT213537 NZ_CP109614:833709-833975 [Klebsiella sp. KP20-425-1]
ATGGACATTAACAATAAAGCACGTATCCACTGGGCATGCCGCCGCGGGATGCGCGAACTCGACATCTCCATCATGCCGTT
CTTTGAGTATGAGTACGATACCCTCAGCGACGCTGACAAACAGCTGTTCATTCGCCTGCTGGAAAATGACGATCCGGATC
TGTTCAACTGGCTGATGAACCACGGCAAACCGGCCGATGCGGAGCTGCAGCGGATGGTAACCTTAATTCAGACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA