Detailed information of TA system TA213536

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 5422..6065
• Replicon: plasmid pAR8538_4
• Accession: NZ_CP081830
• Organism: Klebsiella quasipneumoniae strain AR8538

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: K5P22_RS27545
• Protein ID: WP_114690528.1
• Coordinates: 5649..6065 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: K5P22_RS27540
• Protein ID: WP_001261276.1
• Coordinates: 5422..5652 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5P22_RS27515	1527..2153	+	627	WP_004197649.1	ParA family plasmid-partitioning AAA ATPase	-
K5P22_RS27520	2150..2452	+	303	WP_004197636.1	hypothetical protein	-
K5P22_RS27525	2916..3707	-	792	WP_032667567.1	site-specific integrase	-
K5P22_RS27530	3704..4447	-	744	WP_004098973.1	hypothetical protein	-
K5P22_RS27535	4498..4848	-	351	WP_000493378.1	hypothetical protein	-
K5P22_RS27540	5422..5652	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K5P22_RS27545	5649..6065	+	417	WP_114690528.1	type II toxin-antitoxin system VapC family toxin	Toxin
K5P22_RS27550	6416..7384	-	969	WP_004099053.1	IS5-like element IS903B family transposase	-
K5P22_RS27555	7418..7555	-	138	Protein_9	sugar dehydrogenase	-
K5P22_RS27560	7621..8322	-	702	WP_008502228.1	DsbA family oxidoreductase	-
K5P22_RS27565	8388..9494	-	1107	WP_023287189.1	alkene reductase	-
K5P22_RS27570	9708..10037	+	330	WP_002431133.1	thioredoxin family protein	-
K5P22_RS27575	10067..10405	-	339	WP_000888080.1	carboxymuconolactone decarboxylase family protein	-
K5P22_RS27580	10410..10991	-	582	WP_000210409.1	TetR/AcrR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	aph(6)-Id / aph(3'')-Ib / blaDHA-1 / sul1 / blaNDM-1	htpB	1..55362	55362

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15181.66 Da
• Isoelectric Point: 8.5404

>T213536 WP_114690528.1 NZ_CP081830:5649-6065 [Klebsiella quasipneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRNQRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T213536 NZ_CP109614:356518-356865 [Klebsiella sp. KP20-425-1]
ATGTGGGATGTGGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCTTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTTTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGCGGGCAATAAGGATGGCATAAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT213536 WP_001261276.1 NZ_CP081830:5422-5652 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT213536 NZ_CP109614:356865-357164 [Klebsiella sp. KP20-425-1]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGAAAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGACTGATAACGACCCTCGGCTTTCGACCCTAAAAAGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A387K023