Detailed information of TA system TA213535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 58754..59397
• Replicon: plasmid pAR8538_1
• Accession: NZ_CP081828
• Organism: Klebsiella quasipneumoniae strain AR8538

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: K5P22_RS25735
• Protein ID: WP_049014623.1
• Coordinates: 58981..59397 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: K5P22_RS25730
• Protein ID: WP_001261276.1
• Coordinates: 58754..58984 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5P22_RS25720	54760..56955	+	2196	WP_049013984.1	DEAD/DEAH box helicase	-
K5P22_RS25725	57275..58435	-	1161	WP_064171855.1	IS30-like element ISCfr4 family transposase	-
K5P22_RS25730	58754..58984	+	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
K5P22_RS25735	58981..59397	+	417	WP_049014623.1	type II toxin-antitoxin system VapC family toxin	Toxin
K5P22_RS25745	62203..63300	-	1098	WP_049015454.1	RepB family plasmid replication initiator protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..231578	231578
	-	inside	IScluster/Tn	-	-	57275..70233	12958

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15098.57 Da
• Isoelectric Point: 8.5403

>T213535 WP_049014623.1 NZ_CP081828:58981-59397 [Klebsiella quasipneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRFAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T213535 NZ_CP109614:330549-330917 [Klebsiella sp. KP20-425-1]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGACAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT213535 WP_001261276.1 NZ_CP081828:58754-58984 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT213535 NZ_CP109614:330331-330552 [Klebsiella sp. KP20-425-1]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A387K023