Detailed information of TA system TA213534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 20263..21173
• Replicon: plasmid pAR8538_1
• Accession: NZ_CP081828
• Organism: Klebsiella quasipneumoniae strain AR8538

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: K5P22_RS25560
• Protein ID: WP_032413375.1
• Coordinates: 20703..21173 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7H4PRG4
• Locus tag: K5P22_RS25555
• Protein ID: WP_032413443.1
• Coordinates: 20263..20706 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5P22_RS25520	16097..17095	+	999	WP_032413381.1	N(2)-acetyl-L-2,4-diaminobutanoate deacetylase DoeB	-
K5P22_RS25525	17142..18320	+	1179	WP_032413379.1	cystathionine gamma-synthase family protein	-
K5P22_RS25530	18457..18804	+	348	WP_032413377.1	hypothetical protein	-
K5P22_RS25535	18862..18987	+	126	Protein_20	transposase	-
K5P22_RS25540	19121..19345	-	225	Protein_21	transposase	-
K5P22_RS25545	19365..19550	+	186	WP_071571073.1	DUF2188 domain-containing protein	-
K5P22_RS25550	19651..20163	+	513	Protein_23	DUF4113 domain-containing protein	-
K5P22_RS25555	20263..20706	+	444	WP_032413443.1	DUF2384 domain-containing protein	Antitoxin
K5P22_RS25560	20703..21173	+	471	WP_032413375.1	RES family NAD+ phosphorylase	Toxin
K5P22_RS25565	21283..21543	-	261	WP_017901409.1	hypothetical protein	-
K5P22_RS25570	22115..22492	-	378	Protein_27	integrase core domain-containing protein	-
K5P22_RS25575	22626..23594	+	969	WP_162887377.1	IS5 family transposase	-
K5P22_RS25580	23829..25562	-	1734	WP_004118832.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..231578	231578
	-	inside	IScluster/Tn	-	-	18832..23594	4762

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17547.79 Da
• Isoelectric Point: 4.5152

>T213534 WP_032413375.1 NZ_CP081828:20703-21173 [Klebsiella quasipneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIAFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T213534 NZ_CP109613:c47990-47127 [Enterococcus faecalis]
ATGGCAAATATAGTCAATTTTACTGACAAACAGTTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGGTTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGAAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTGTTGTCATTGATAATGATACCTTCAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTCTATGAAAAAGACGTAGTAAAACATGCAACACCTTATTCTAATCGTATGACAGAAGCACTTATTAGCCGTTTAAG
CGATCAAGGCTATAATTTAGTGATTGAAGGCACAGGACGAACAACAGACGTTCCTATTCAAACAGCCACAATGCTACAAG
CCAAAGGTTATGAAACAAAAACGTACGCCATGGCAGTACCCAAAATTGAATCATACTTAGGAACGATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCTAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTTAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16508.82 Da
• Isoelectric Point: 10.3013

>AT213534 WP_032413443.1 NZ_CP081828:20263-20706 [Klebsiella quasipneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT213534 NZ_CP109613:c48264-47992 [Enterococcus faecalis]
ATGGCAGTTACGTATGAAAAAACTTTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAATCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTAAAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A7H4PRG4