Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4062758..4063377 Replicon chromosome
Accession NZ_CP081827
Organism Klebsiella quasipneumoniae strain AR8538

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K5P22_RS19565 Protein ID WP_002892050.1
Coordinates 4063159..4063377 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K5P22_RS19560 Protein ID WP_002892066.1
Coordinates 4062758..4063132 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5P22_RS19550 (4057911) 4057911..4059104 + 1194 WP_023288645.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K5P22_RS19555 (4059127) 4059127..4062273 + 3147 WP_023288644.1 multidrug efflux RND transporter permease subunit AcrB -
K5P22_RS19560 (4062758) 4062758..4063132 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K5P22_RS19565 (4063159) 4063159..4063377 + 219 WP_002892050.1 HHA domain-containing protein Toxin
K5P22_RS19570 (4063536) 4063536..4064102 + 567 WP_023288643.1 maltose O-acetyltransferase -
K5P22_RS19575 (4064074) 4064074..4064205 - 132 WP_162543094.1 hypothetical protein -
K5P22_RS19580 (4064239) 4064239..4064709 + 471 WP_064155186.1 YlaC family protein -
K5P22_RS19585 (4064678) 4064678..4066135 - 1458 WP_087824767.1 PLP-dependent aminotransferase family protein -
K5P22_RS19590 (4066236) 4066236..4066934 + 699 WP_044521289.1 GNAT family protein -
K5P22_RS19595 (4066931) 4066931..4067071 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
K5P22_RS19600 (4067071) 4067071..4067334 - 264 WP_087824768.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T213532 WP_002892050.1 NZ_CP081827:4063159-4063377 [Klebsiella quasipneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T213532 NZ_CP109611:2680674-2680814 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAATAAAAAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT213532 WP_002892066.1 NZ_CP081827:4062758-4063132 [Klebsiella quasipneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT213532 NZ_CP109611:c2681006-2680747 [Enterococcus faecalis]
ATAAAAATGCCCGAGCCTCTTATTTTTCTAAGGCTCGGGCATTTTTATTGATGGTCTCTTGAGGGTCGTTGTGCTATAAT
GGTAATGAAAAGAGAGATATGCTGTAACATACCTCTCTAGTGTAGAGCCGTTTAAGACGATAGCGATTTTTAGTGATTTA
TAAAAAATAACCGTGCTCGCTAAAGTTGACGGTTATTTTTTATTGTCATTTTTAAGCAATTTCACAATCAGTGCAATCAA
AGCAATGGTAAACATACCAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References