Detailed information of TA system TA213531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2665450..2667038
• Replicon: chromosome
• Accession: NZ_CP081827
• Organism: Klebsiella quasipneumoniae strain AR8538

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: K5P22_RS12930
• Protein ID: WP_044523012.1
• Coordinates: 2665450..2666772 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A2N4W0X3
• Locus tag: K5P22_RS12935
• Protein ID: WP_023289667.1
• Coordinates: 2666772..2667038 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5P22_RS12915 (2660494)	2660494..2663007	+	2514	WP_249481327.1	fimbria/pilus outer membrane usher protein	-
K5P22_RS12920 (2662995)	2662995..2663999	+	1005	WP_044523017.1	fimbrial protein	-
K5P22_RS12925 (2664141)	2664141..2665433	+	1293	WP_044523014.1	glycoside hydrolase family 10 protein	-
K5P22_RS12930 (2665450)	2665450..2666772	-	1323	WP_044523012.1	type II toxin-antitoxin system HipA family toxin	Toxin
K5P22_RS12935 (2666772)	2666772..2667038	-	267	WP_023289667.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
K5P22_RS12940 (2667302)	2667302..2667628	-	327	WP_249481328.1	hydroxyisourate hydrolase	-
K5P22_RS12945 (2667625)	2667625..2668125	-	501	WP_032452825.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
K5P22_RS12950 (2668103)	2668103..2669482	-	1380	WP_249481330.1	nucleobase:cation symporter-2 family protein	-
K5P22_RS12955 (2669828)	2669828..2670982	+	1155	WP_249481332.1	FAD-dependent urate hydroxylase HpxO	-
K5P22_RS12960 (2670963)	2670963..2671886	-	924	WP_249481334.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49183.49 Da
• Isoelectric Point: 8.6110

>T213531 WP_044523012.1 NZ_CP081827:c2666772-2665450 [Klebsiella quasipneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDDEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLAALRWQTLDEAQLTALLTAYQSDIPLGMVTGQDDFRISVAGAQEKTAL
LRMGEHWCIPQGTTPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARALGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGIPSSMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSLFLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATRGRKTEINAIYPRHFLATAKAVNFPRDQMLAILDEFADRVP
QAIESARRTLPADFSAHVWRAITENMLKLHARLQQGLQAR

Nucleotide

• Length: 1323 bp

>T213531 NZ_CP109611:2680674-2680814 [Enterococcus faecalis]
ATTTCTCTGAAAAATACGAATAAAAATATCGTTTACTGTACAACATATGAAACAATTCAGACGATCCTAGGGTTTGGTAT
GTTTACCATTGCTTTGATTGCACTGATTGTGAAATTGCTTAAAAATGACAATAAAAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9891.25 Da
• Isoelectric Point: 7.3163

>AT213531 WP_023289667.1 NZ_CP081827:c2667038-2666772 [Klebsiella quasipneumoniae]
MHSPQIYSPVQLANYLKLLRQQNHWTQEALASRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKPAETATAP
DNTTDLDW

Nucleotide

• Length: 267 bp

>AT213531 NZ_CP109611:c2680672-2680529 [Enterococcus faecalis]
TGCTACAATAGAGACGAAAAGAGAGGTATGCTCTAACATACCTCTCTAGTGTAGAGCCGTTTCAGACGGTGACCTTTTTA
GTTACAAAAAATAACCGTACTCAGTCAAAGTAGACGGTTATTTTTTCTTGTCATTTTTAAGCAA

Structures

Antitoxin

• AlphaFold DB: A0A2N4W0X3