Detailed information of TA system TA213522

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 856048..856852
• Replicon: chromosome
• Accession: NZ_CP081827
• Organism: Klebsiella quasipneumoniae strain AR8538

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: K5P22_RS04245
• Protein ID: WP_099144045.1
• Coordinates: 856463..856852 (+)
• Length: 130 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: K5P22_RS04240
• Protein ID: WP_099144044.1
• Coordinates: 856048..856407 (+)
• Length: 120 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5P22_RS04205 (851358)	851358..851993	-	636	WP_099144040.1	inovirus Gp2 family protein	-
K5P22_RS04210 (852631)	852631..853452	+	822	WP_099144041.1	hypothetical protein	-
K5P22_RS04215 (853696)	853696..854520	+	825	WP_099144042.1	DUF932 domain-containing protein	-
K5P22_RS04220 (854544)	854544..854774	+	231	WP_097365535.1	DUF905 domain-containing protein	-
K5P22_RS04225 (854854)	854854..855297	+	444	WP_046876932.1	antirestriction protein	-
K5P22_RS04230 (855309)	855309..855788	+	480	WP_046876931.1	DNA repair protein RadC	-
K5P22_RS04235 (855802)	855802..856023	+	222	WP_099144043.1	DUF987 domain-containing protein	-
K5P22_RS04240 (856048)	856048..856407	+	360	WP_099144044.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
K5P22_RS04245 (856463)	856463..856852	+	390	WP_099144045.1	TA system toxin CbtA family protein	Toxin
K5P22_RS04250 (856939)	856939..857778	+	840	WP_099144046.1	DUF4942 domain-containing protein	-
K5P22_RS04255 (858119)	858119..859507	-	1389	WP_044524670.1	MFS transporter	-
K5P22_RS04260 (859637)	859637..860554	+	918	WP_171878670.1	LysR family transcriptional regulator	-
K5P22_RS04265 (860802)	860802..861503	-	702	Protein_837	carboxymuconolactone decarboxylase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	mrkJ / mrkF / mrkD / mrkC / mrkB	836940..857778	20838

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (3-111)

Antitoxin

• Antitoxin CbeA (15-114)

Sequences

Toxin

Protein

• Length: 130 a.a.
• Molecular weight: 14487.61 Da
• Isoelectric Point: 8.9843

>T213522 WP_099144045.1 NZ_CP081827:856463-856852 [Klebsiella quasipneumoniae]
MQTKSPPFMRAASSRPSPVGVWQTLLTCLLEHHYGLTLNDTPFSDELVIQQHIEAGISLADALNFIVEKYELVRTDRPGF
SIREQSPFITPIDILRARKATGLMNRDTYKEVTAITRGQHPQVSAPGKR

Nucleotide

• Length: 390 bp

>T213522 NZ_CP109611:c75056-74874 [Enterococcus faecalis]
ATGCCAATGACACAAAAACAAATGGTCAAATTGCTAAAAAAGCACGGTTGGTTACCAACCGATGGTGGAAAAGGCTCCCA
TATAAAAATGGAGATGTCTGGCAAAAGACCCATCATTATCCCACACGGAGAATTGAAAAAAGGAACCGAAATAGGGATTT
TAAAAGAGGCAGGGCTGAAATAG

Antitoxin

Protein

• Length: 120 a.a.
• Molecular weight: 13466.34 Da
• Isoelectric Point: 6.6256

>AT213522 WP_099144044.1 NZ_CP081827:856048-856407 [Klebsiella quasipneumoniae]
MSKKTTITTHDISEPWWGLRRSVSPCFGARLVQEGNRLYYLADRASFDGQFCDADLRHLDQAFPVLMKQLELMLTSGELN
PRHQHCVTLYAKGLTCEADTLGSHGYVYIAIYPTPVTTE

Nucleotide

• Length: 360 bp

>AT213522 NZ_CP109611:c74840-74403 [Enterococcus faecalis]
ATGCTAATTAGTTATCCTGCATGTTTCTACAGTGTCGAGAATGGTTACTATGTTTATTTCCCAGATATAGGTGGTTCTGG
TACGCAAGGAGATACAATTCCTGATGCAATTGCGATGGCTTCCGATTATTTAGGTATGATGTTATCTGATGATATTGAAC
ATCAAAGAACAATCCACCAGCCATCTCCTATCAATACGTTGTCATTAGAAGATAATAATCCCTTTAGAGATGACGATGAC
TTTAATTTTAACCTTGCAGACTCGTTTGTTTCTATGGTAAATGTAGAACTAAATGATTATTTAGGAACAAATACGTTAGT
CAAAAAAACCGTCACCATTCCCAAGTGGTCTGATGAACTCGGGAAAAAAAATAAATTGAATTTTTCTAAAACCTTGACCG
ATGCCATCGTTGAAAAGTCGCTACATGTTTTAAAGTAG