Detailed information of TA system TA213518

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3449925..3450560
• Replicon: chromosome
• Accession: NZ_CP081825
• Organism: Paenibacillus sp. DR312

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: W4B4V0
• Locus tag: K5K90_RS14950
• Protein ID: WP_024630714.1
• Coordinates: 3450210..3450560 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: W4B5R5
• Locus tag: K5K90_RS14945
• Protein ID: WP_017690037.1
• Coordinates: 3449925..3450206 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5K90_RS14930 (K5K90_14930)	3445141..3446634	-	1494	WP_222215976.1	serine hydrolase	-
K5K90_RS14935 (K5K90_14935)	3446883..3448082	+	1200	WP_091015327.1	outer membrane lipoprotein-sorting protein	-
K5K90_RS14940 (K5K90_14940)	3448478..3449665	+	1188	WP_222215977.1	alanine racemase	-
K5K90_RS14945 (K5K90_14945)	3449925..3450206	+	282	WP_017690037.1	ribbon-helix-helix protein, CopG family	Antitoxin
K5K90_RS14950 (K5K90_14950)	3450210..3450560	+	351	WP_024630714.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
K5K90_RS14955 (K5K90_14955)	3450796..3452985	+	2190	WP_222215978.1	alpha-galactosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-46)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12778.77 Da
• Isoelectric Point: 5.1184

>T213518 WP_024630714.1 NZ_CP081825:3450210-3450560 [Paenibacillus sp. DR312]
MIVKRGDVFFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTCIVAAITAQIQKAKLPTHVEIDAAAHGFDRDSVILLEQI
RTIDKQRLTDKITHLDEETMKLVNEALQISLGLIDF

Nucleotide

• Length: 351 bp

>T213518 NZ_CP109607:c5249992-5249609 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10737.36 Da
• Isoelectric Point: 5.8649

>AT213518 WP_017690037.1 NZ_CP081825:3449925-3450206 [Paenibacillus sp. DR312]
VANLQNTKRIMISLPDYLLQEVDGIVALENSNRSELIRQAMKLYLTERKKRYIRETMQRGYMEMAKINLTMASEAFHAEE
DADSTLDRLVSGV

Nucleotide

• Length: 282 bp

>AT213518 NZ_CP109607:c5250234-5249992 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: W4B4V0

Antitoxin

• AlphaFold DB: W4B5R5