Detailed information of TA system TA213516

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relB-parE/ParE-RHH
• Location: 1592..2172
• Replicon: plasmid pF726925-3
• Accession: NZ_CP081823
• Organism: Klebsiella pneumoniae strain F726925

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A8J3DTL7
• Locus tag: K5O13_RS28500
• Protein ID: WP_071177730.1
• Coordinates: 1858..2172 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2X1PRM1
• Locus tag: K5O13_RS28495
• Protein ID: WP_000093040.1
• Coordinates: 1592..1870 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5O13_RS28480 (K5O13_28480)	165..410	+	246	WP_032440458.1	hypothetical protein	-
K5O13_RS28485 (K5O13_28485)	684..1055	+	372	WP_001237044.1	cell envelope integrity protein TolA	-
K5O13_RS28860	1052..1417	+	366	WP_072354022.1	TonB family protein	-
K5O13_RS28495 (K5O13_28495)	1592..1870	+	279	WP_000093040.1	CopG family ribbon-helix-helix protein	Antitoxin
K5O13_RS28500 (K5O13_28500)	1858..2172	+	315	WP_071177730.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
K5O13_RS28505 (K5O13_28505)	2336..2764	+	429	WP_001140599.1	hypothetical protein	-
K5O13_RS28510 (K5O13_28510)	2790..2969	+	180	WP_000165970.1	Rop family plasmid primer RNA-binding protein	-
K5O13_RS28515 (K5O13_28515)	2996..3526	-	531	WP_071177729.1	hypothetical protein	-
K5O13_RS28520 (K5O13_28520)	3533..4264	-	732	WP_071177728.1	MobC family replication-relaxation protein	-
K5O13_RS28525 (K5O13_28525)	4264..6228	-	1965	WP_071177727.1	TraM recognition domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..10060	10060

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-96)

Antitoxin

• Ribbon-helix-helix protein, CopG (5-42)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11805.73 Da
• Isoelectric Point: 9.8324

>T213516 WP_071177730.1 NZ_CP081823:1858-2172 [Klebsiella pneumoniae]
MPQVTISALAQRDLQRLQDFLKTKNRLAARKGGEVIVRAIQQLKTLPDIGRPVPFLPLEYKELVIGFGDSGYVMLYRHDR
EMDQIVIVTVRHQKESGYPGADSL

Nucleotide

• Length: 315 bp

>T213516 NZ_CP109607:c4769659-4769375 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10709.73 Da
• Isoelectric Point: 4.7561

>AT213516 WP_000093040.1 NZ_CP081823:1592-1870 [Klebsiella pneumoniae]
MSSATSIRLDEELKDRLKTLADDRHRSAHALMLEAITEYIDREEKRSQYLRDGQAAWQHYQETGLHLTAEEAEAWISTWG
TENEQDAPPCHR

Nucleotide

• Length: 279 bp

>AT213516 NZ_CP109607:c4769891-4769649 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A2X1PRM1