Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 56678..57321 Replicon plasmid pF726925-1
Accession NZ_CP081821
Organism Klebsiella pneumoniae strain F726925

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag K5O13_RS27300 Protein ID WP_001044770.1
Coordinates 56905..57321 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag K5O13_RS27295 Protein ID WP_001261282.1
Coordinates 56678..56908 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O13_RS27260 (51901) 51901..52680 - 780 WP_013214009.1 site-specific integrase -
K5O13_RS27265 (52864) 52864..53868 - 1005 WP_011977814.1 hypothetical protein -
K5O13_RS27270 (53898) 53898..54101 - 204 WP_011977813.1 hypothetical protein -
K5O13_RS27275 (54147) 54147..54668 - 522 WP_013214008.1 hypothetical protein -
K5O13_RS27280 (54726) 54726..55118 - 393 WP_011977811.1 hypothetical protein -
K5O13_RS27285 (55155) 55155..56099 - 945 WP_011977810.1 hypothetical protein -
K5O13_RS27290 (56260) 56260..56721 - 462 WP_014343465.1 hypothetical protein -
K5O13_RS27295 (56678) 56678..56908 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K5O13_RS27300 (56905) 56905..57321 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
K5O13_RS27305 (57395) 57395..58957 + 1563 WP_004206609.1 AAA family ATPase -
K5O13_RS27310 (58942) 58942..59964 + 1023 WP_000361404.1 helicase UvrD -
K5O13_RS27315 (60220) 60220..60917 + 698 WP_223174630.1 IS1 family transposase -
K5O13_RS27320 (60946) 60946..61218 + 273 Protein_86 transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaKPC-2 / rmtB / blaTEM-1B / fosA3 / blaCTX-M-65 / catA2 - 1..172862 172862


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T213508 WP_001044770.1 NZ_CP081821:56905-57321 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T213508 NZ_CP109607:324618-324986 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT213508 WP_001261282.1 NZ_CP081821:56678-56908 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT213508 NZ_CP109607:324400-324621 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References