Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5382213..5382838 Replicon chromosome
Accession NZ_CP081820
Organism Klebsiella pneumoniae strain F726925

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K5O13_RS26545 Protein ID WP_002882817.1
Coordinates 5382213..5382596 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K5O13_RS26550 Protein ID WP_004150355.1
Coordinates 5382596..5382838 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O13_RS26530 (K5O13_26530) 5379579..5380481 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K5O13_RS26535 (K5O13_26535) 5380478..5381113 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K5O13_RS26540 (K5O13_26540) 5381110..5382039 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K5O13_RS26545 (K5O13_26545) 5382213..5382596 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K5O13_RS26550 (K5O13_26550) 5382596..5382838 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K5O13_RS26555 (K5O13_26555) 5383043..5383960 + 918 WP_002882812.1 alpha/beta hydrolase -
K5O13_RS26560 (K5O13_26560) 5383974..5384915 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K5O13_RS26565 (K5O13_26565) 5384960..5385397 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K5O13_RS26570 (K5O13_26570) 5385394..5386254 - 861 WP_002882807.1 virulence factor BrkB family protein -
K5O13_RS26575 (K5O13_26575) 5386248..5386847 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T213507 WP_002882817.1 NZ_CP081820:c5382596-5382213 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T213507 NZ_CP109606:4579323-4579616 [Pseudomonas putida]
ATGACTGAAACCTTTTCCGAATTCGACCCCGCCGAGTACCTCACAACGCCCGAAGCAATCGCGCAGTTCATGACCGACGC
ACTTGAGACTGGCGATGCCGGCTATGTTGCCAAAGCGGTTGGCGTGGTGGCCCGTGCAAAGGGCATGAGCGAACTGGCCA
AAGAAACTGGCCTGTCTCGCGAACAGCTCTACCGCTCTTTCAGCGAGCACGGCAACCCGACCCTGAAGTCGTTCCTGACC
GTAATGAAGGCATTAGGCATAGACATGACGGCGCGCCCCCACCTTGCTGGCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT213507 WP_004150355.1 NZ_CP081820:c5382838-5382596 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT213507 NZ_CP109606:4579028-4579330 [Pseudomonas putida]
ATGAAGAAAATCGAATCCAGCAGTTTCAGACATTGGGTGACCGGCCTGCGGGACAGCAGCGCCAGAGCCCGCATCATTTC
TCGGATCAACCGGTTAATGGAGGGTTTACCTGGCGACGTATCTCCGGTTGGCCATGGCGTCAGTGAACTCAGGATTCACT
ACGGGCCTGGCTACCGGGTGTACTTCCATCAGACGGGCAACACTTTCGTCATTCTGTTATGTAGTGGCGACAAAAGCAGC
CAACAGCGTGACATCAAGGCAGCCCACCAGATTCTGCGTTCGTGGAGGATGCAAAATGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References