Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4185980..4186599 Replicon chromosome
Accession NZ_CP081820
Organism Klebsiella pneumoniae strain F726925

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K5O13_RS20805 Protein ID WP_002892050.1
Coordinates 4186381..4186599 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K5O13_RS20800 Protein ID WP_002892066.1
Coordinates 4185980..4186354 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O13_RS20790 (K5O13_20790) 4181132..4182325 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K5O13_RS20795 (K5O13_20795) 4182348..4185494 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
K5O13_RS20800 (K5O13_20800) 4185980..4186354 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K5O13_RS20805 (K5O13_20805) 4186381..4186599 + 219 WP_002892050.1 HHA domain-containing protein Toxin
K5O13_RS20810 (K5O13_20810) 4186758..4187324 + 567 WP_002892030.1 maltose O-acetyltransferase -
K5O13_RS20815 (K5O13_20815) 4187296..4187436 - 141 WP_004147370.1 hypothetical protein -
K5O13_RS20820 (K5O13_20820) 4187457..4187927 + 471 WP_002892026.1 YlaC family protein -
K5O13_RS20825 (K5O13_20825) 4187902..4189353 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
K5O13_RS20830 (K5O13_20830) 4189454..4190152 + 699 WP_002892021.1 GNAT family protein -
K5O13_RS20835 (K5O13_20835) 4190149..4190289 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
K5O13_RS20840 (K5O13_20840) 4190289..4190552 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T213503 WP_002892050.1 NZ_CP081820:4186381-4186599 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T213503 NZ_CP109606:c4093052-4092477 [Pseudomonas putida]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAATCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACAGGGCCATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTACCCGATCCGAACGATCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGTGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTGCTGGATCTGGATGCGGCCGCCGTAGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGATGTGGACCGCTATCTCGAAATCCTGCTGCGCCAAGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT213503 WP_002892066.1 NZ_CP081820:4185980-4186354 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT213503 NZ_CP109606:c4093519-4093049 [Pseudomonas putida]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTTAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTAGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References