Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2742747..2744335 Replicon chromosome
Accession NZ_CP081820
Organism Klebsiella pneumoniae strain F726925

Toxin (Protein)


Gene name hipA Uniprot ID A0A0H3GN97
Locus tag K5O13_RS13565 Protein ID WP_004199851.1
Coordinates 2742747..2744069 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag K5O13_RS13570 Protein ID WP_002904790.1
Coordinates 2744069..2744335 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O13_RS13550 (K5O13_13550) 2737812..2740313 + 2502 WP_004199853.1 fimbria/pilus outer membrane usher protein -
K5O13_RS13555 (K5O13_13555) 2740301..2741305 + 1005 WP_004199852.1 fimbrial protein -
K5O13_RS13560 (K5O13_13560) 2741438..2742730 + 1293 WP_002904794.1 glycoside hydrolase family 10 protein -
K5O13_RS13565 (K5O13_13565) 2742747..2744069 - 1323 WP_004199851.1 type II toxin-antitoxin system HipA family toxin Toxin
K5O13_RS13570 (K5O13_13570) 2744069..2744335 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
K5O13_RS13575 (K5O13_13575) 2744601..2744927 - 327 WP_002904788.1 hydroxyisourate hydrolase -
K5O13_RS13580 (K5O13_13580) 2744924..2745424 - 501 WP_002904787.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
K5O13_RS13585 (K5O13_13585) 2745402..2746781 - 1380 WP_004199850.1 nucleobase:cation symporter-2 family protein -
K5O13_RS13590 (K5O13_13590) 2747127..2748281 + 1155 WP_002904783.1 FAD-dependent urate hydroxylase HpxO -
K5O13_RS13595 (K5O13_13595) 2748262..2749185 - 924 WP_004199849.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-402)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49128.44 Da        Isoelectric Point: 7.2487

>T213502 WP_004199851.1 NZ_CP081820:c2744069-2742747 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPLEQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T213502 NZ_CP109606:2227036-2227473 [Pseudomonas putida]
GTGATTCTGTGGCGTATCAGCGCCTACGCAGACTTGAGCGGCACGGGGGGCTTGCGGGTAAGCGGCCGTTGGCACCAGGC
GGGCCGGCCGGTGGTATATGCCGCCACCAGCCCTCCAGGGGCGATGCTCGAGGTGTTGGTGCACCTGGAGATCGACCCTG
AGGATTTCCCCACTACCCTGCGCCTGATCCGCATCGAATTGCCCGACACGGTTTCACAAGCGCGGTTACCCACCTTGCAG
CCGGGCTGGTCCGCTCAGCCGGAGTTGACCCGGGCACTGGGCAATCGCTTTCTGGATGACTGTTCGGCATTGTTGTTGCC
GGTGCCGAGCGCGATCATGCCCAGTACCACCAACTACCTGTTCAACCCACGGCACCCGCAGGCGCAGAGCGCGAAAATAC
AGGTCGAGGATTTCACCCCGGACAGCCGCTTGTTCTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT213502 WP_002904790.1 NZ_CP081820:c2744335-2744069 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT213502 NZ_CP109606:2226590-2227039 [Pseudomonas putida]
ATGCTTGCCGAAGTGCTTCGCGACAACGGTTACCATGAGTACCGGGCGCGCTTGAACGCGCTGCTGGAAATCCCCGAGCT
TGCCAGTGACTTTGAAATCCACACCCGCATCACCCATGGTTTTGCCGCCACCTGGCTGGTAAAGCTGACCGAGCGTGGTG
TGCTCACGCCGGTGGAGCGCGACCAGATCATTCCCCTGCGCACCCTGAAAACCCGCATTGAGCGCGACCAGCCGTTGACC
GTGGATGAAAGCGACCGTCTGTTCCGCTCGGCCCACATCACCGCCATGGCCGAAGCCGTGTTCGGCGAGGCGGACAAGGC
CAAACGTTGGTTGTCCAAGCCCAAGGAGCGCTTTTCGGGGCTCACCCCGATGCAGATGCTTACCACCCAGCAAGGCACCA
CTCAGGTCGAAGAGATGCTGCTACAGATTGCCGAGGGCTTTGGCCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GN97

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References