Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 7321..7964 Replicon plasmid p2
Accession NZ_CP081818
Organism Klebsiella pneumoniae strain AR8416

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag K5O16_RS26705 Protein ID WP_000754566.1
Coordinates 7321..7737 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag K5O16_RS26710 Protein ID WP_001261276.1
Coordinates 7734..7964 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O16_RS26680 3088..4017 + 930 Protein_5 transposase -
K5O16_RS26685 4180..4263 - 84 Protein_6 SOS response-associated peptidase -
K5O16_RS26690 4783..4884 + 102 Protein_7 Tn3 family transposase -
K5O16_RS26695 4904..5979 - 1076 Protein_8 IS110 family transposase -
K5O16_RS26700 6261..7117 - 857 Protein_9 IS3-like element ISEc15 family transposase -
K5O16_RS26705 7321..7737 - 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
K5O16_RS26710 7734..7964 - 231 WP_001261276.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
K5O16_RS26715 8538..8888 + 351 WP_000493378.1 hypothetical protein -
K5O16_RS26720 8939..9682 + 744 WP_000129823.1 hypothetical protein -
K5O16_RS26725 9679..10455 + 777 WP_000015958.1 site-specific integrase -
K5O16_RS26730 10513..10770 - 258 WP_000764642.1 hypothetical protein -
K5O16_RS26735 10899..11003 - 105 WP_032409716.1 hypothetical protein -
K5O16_RS26740 11533..12399 + 867 WP_004118283.1 replication initiation protein -
K5O16_RS26745 12576..12845 - 270 WP_000339857.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aph(6)-Id / qnrS1 / aph(3'')-Ib / sul2 / tet(A) / floR - 1..50175 50175


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T213489 WP_000754566.1 NZ_CP081818:c7737-7321 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T213489 NZ_CP109604:324330-324698 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGTCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTATAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT213489 WP_001261276.1 NZ_CP081818:c7964-7734 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT213489 NZ_CP109604:324112-324333 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGCTCAGGGAGCTTGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References