Detailed information of TA system TA213488

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 5081664..5083252
• Replicon: chromosome
• Accession: NZ_CP081816
• Organism: Klebsiella pneumoniae strain AR8416

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A485W5A2
• Locus tag: K5O16_RS25670
• Protein ID: WP_023342812.1
• Coordinates: 5081664..5082986 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: K5O16_RS25675
• Protein ID: WP_024622638.1
• Coordinates: 5082986..5083252 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5O16_RS25655	5076720..5079221	+	2502	WP_023342811.1	fimbria/pilus outer membrane usher protein	-
K5O16_RS25660	5079209..5080213	+	1005	WP_004224758.1	fimbrial protein	-
K5O16_RS25665	5080355..5081647	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
K5O16_RS25670	5081664..5082986	-	1323	WP_023342812.1	type II toxin-antitoxin system HipA family toxin	Toxin
K5O16_RS25675	5082986..5083252	-	267	WP_024622638.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
K5O16_RS25680	5083517..5083843	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
K5O16_RS25685	5083840..5084340	-	501	WP_020804988.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
K5O16_RS25690	5084318..5085697	-	1380	WP_048292036.1	nucleobase:cation symporter-2 family protein	-
K5O16_RS25695	5086043..5087197	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
K5O16_RS25700	5087178..5088101	-	924	WP_040089016.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49171.46 Da
• Isoelectric Point: 7.5358

>T213488 WP_023342812.1 NZ_CP081816:c5082986-5081664 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEVNAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T213488 NZ_CP109603:c5466661-5466086 [Pseudomonas putida]
ATGAGGCAGTCACCCTTTACTGTTGTCTATGATGCCTGCGTGCTGTATCCCGCGCCCTTGCGTGATCTACTGATGCATCT
GGCTCTGACAGGCGTGTATCGCGCGCGCTGGTCCGAGCAAATTCACGATGAATGGACGCGAAACGTGTTGAAAAACCGCC
CTGACATAGCGGCTGCACAACTTGCTCGCACAGTCGCATTCATGAATCAGGCTGTTGCTGACTCCTTGGTGAGCGACTAC
GAGCCGTTGATACAAGGGCTAGACCTTCCTGACGAAGACGATAGACACGTCTTGGCGGCAGCCATCAAATGTGGCGCCAC
AGTGATCGTCACATACAACCTCAAAGATTTCCCGCCCACGATCTTGAAACGTTTTGAGATCGAAGCATTGCATCCCGACG
TGTTCCTTTCCGACATTTGGGACCTTGACCAAGCAGCCGTGCTCGAGGCCGTCAAGAAGCAGCGCGTGGCGCTGAAGAAC
CCTGTTTATACACCCCGCGAGCTACTCGATACGCTCCTCAAGCAGCGACTCCCAGAGATCGTCAAGCAACTCTCGAATTA
CGAACTGTTGATCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9924.30 Da
• Isoelectric Point: 8.5912

>AT213488 WP_024622638.1 NZ_CP081816:c5083252-5082986 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYMKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT213488 NZ_CP109603:c5467125-5466661 [Pseudomonas putida]
ATGACCACCGCCGACCTCGCTCAAACCATCCTCCCCGATGAAAAAGCGATCGCTGCGGCTATTGAGTCAAGCCGCCAGAT
TGCCGCTTTCCTCTCGACGAAGCTGGATACTCAGCGCATTGAGCTCGTTGATGAAGCCCAGCAACGTCAGACCGTTGAAC
TGCCAACGTTCGCGCTCAGACTGCTCGGCGACATACTTAACGAACTGGCCTTGGGCAACACCGTAAAGGTGGTGCCTATT
CATGCAGAACTGACCACTCAAGAAGGCGCAGACATGCTCAACGTCTCGCGCCCTCACCTGGTCAAGCTTCTAGACGAGGG
GCTAATCCCACATACCAAAACCGGCAGCCACCGCCGCATAAAGTTCACCGACCTGATGGCTTACAAGGCTGAGCGTGATA
AAGCGAGCCAGGCCGCCATGGAAGCACTGGCTGCACAAGCCCAAGAACTCAATATGGGGTATTGA

Structures

Toxin

• AlphaFold DB: A0A485W5A2