Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2382255..2382880 Replicon chromosome
Accession NZ_CP081816
Organism Klebsiella pneumoniae strain AR8416

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag K5O16_RS12360 Protein ID WP_002882817.1
Coordinates 2382255..2382638 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag K5O16_RS12365 Protein ID WP_004150355.1
Coordinates 2382638..2382880 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O16_RS12345 2379621..2380523 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
K5O16_RS12350 2380520..2381155 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
K5O16_RS12355 2381152..2382081 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
K5O16_RS12360 2382255..2382638 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
K5O16_RS12365 2382638..2382880 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
K5O16_RS12370 2383085..2384002 + 918 WP_023302328.1 alpha/beta hydrolase -
K5O16_RS12375 2384016..2384957 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
K5O16_RS12380 2385002..2385439 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
K5O16_RS12385 2385436..2386296 - 861 WP_004146232.1 virulence factor BrkB family protein -
K5O16_RS12390 2386290..2386889 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T213476 WP_002882817.1 NZ_CP081816:c2382638-2382255 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T213476 NZ_CP109602:c44603-44454 [Escherichia coli]
ATGACGAAATATGCCCTTATCGGGGTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTTACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT213476 WP_004150355.1 NZ_CP081816:c2382880-2382638 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT213476 NZ_CP109602:44647-44708 [Escherichia coli]
TTAAGGTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References