Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1187153..1187772 Replicon chromosome
Accession NZ_CP081816
Organism Klebsiella pneumoniae strain AR8416

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag K5O16_RS06660 Protein ID WP_002892050.1
Coordinates 1187554..1187772 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag K5O16_RS06655 Protein ID WP_002892066.1
Coordinates 1187153..1187527 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O16_RS06645 1182305..1183498 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K5O16_RS06650 1183521..1186667 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
K5O16_RS06655 1187153..1187527 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
K5O16_RS06660 1187554..1187772 + 219 WP_002892050.1 HHA domain-containing protein Toxin
K5O16_RS06665 1187931..1188497 + 567 WP_002892030.1 maltose O-acetyltransferase -
K5O16_RS06670 1188469..1188609 - 141 WP_004147370.1 hypothetical protein -
K5O16_RS06675 1188630..1189100 + 471 WP_002892026.1 YlaC family protein -
K5O16_RS06680 1189075..1190526 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
K5O16_RS06685 1190627..1191325 + 699 WP_002892021.1 GNAT family protein -
K5O16_RS06690 1191322..1191462 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
K5O16_RS06695 1191462..1191725 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T213474 WP_002892050.1 NZ_CP081816:1187554-1187772 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T213474 NZ_CP109601:c4260525-4260352 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT213474 WP_002892066.1 NZ_CP081816:1187153-1187527 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT213474 NZ_CP109601:c4260849-4260553 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGATT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References