Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 23528..24264 Replicon plasmid p1
Accession NZ_CP081815
Organism Klebsiella pneumoniae strain AR8416

Toxin (Protein)


Gene name tacT Uniprot ID A0A443XD13
Locus tag K5O16_RS00120 Protein ID WP_075253142.1
Coordinates 23528..24010 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag K5O16_RS00125 Protein ID WP_003026799.1
Coordinates 23998..24264 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O16_RS00105 19396..21108 + 1713 WP_114432437.1 ROK family protein -
K5O16_RS00110 21531..21926 - 396 WP_094645305.1 helix-turn-helix domain-containing protein -
K5O16_RS00115 21975..23321 - 1347 WP_094645307.1 ISNCY family transposase -
K5O16_RS00120 23528..24010 - 483 WP_075253142.1 GNAT family N-acetyltransferase Toxin
K5O16_RS00125 23998..24264 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
K5O16_RS00130 24709..24939 - 231 WP_004206660.1 hypothetical protein -
K5O16_RS00135 24953..25075 - 123 Protein_26 Hha/YmoA family nucleoid-associated regulatory protein -
K5O16_RS00140 25263..26474 - 1212 WP_032415728.1 hypothetical protein -
K5O16_RS00145 26471..26719 - 249 WP_004193994.1 hypothetical protein -
K5O16_RS00150 27077..27427 + 351 WP_004206664.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
K5O16_RS00155 27479..27841 + 363 WP_004206665.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..180897 180897
- flank IS/Tn - - 21975..23333 1358


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17254.90 Da        Isoelectric Point: 8.7400

>T213472 WP_075253142.1 NZ_CP081815:c24010-23528 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T213472 NZ_CP109601:3717160-3717438 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT213472 WP_003026799.1 NZ_CP081815:c24264-23998 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT213472 NZ_CP109601:3716897-3717157 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A443XD13

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References