Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/ParE-DnaT |
| Location | 12891..13413 | Replicon | plasmid unnamed |
| Accession | NZ_CP081814 | ||
| Organism | Shigella flexneri strain WW1 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | G3CAN5 |
| Locus tag | K5O10_RS22975 | Protein ID | WP_000220560.1 |
| Coordinates | 12891..13172 (-) | Length | 94 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | G3CAG1 |
| Locus tag | K5O10_RS22980 | Protein ID | WP_000121743.1 |
| Coordinates | 13162..13413 (-) | Length | 84 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| K5O10_RS22930 (K5O10_22930) | 7900..8391 | - | 492 | WP_038976853.1 | transcription termination/antitermination NusG family protein | - |
| K5O10_RS22935 (K5O10_22935) | 8752..9909 | - | 1158 | WP_000538023.1 | DNA distortion polypeptide 2 | - |
| K5O10_RS22940 (K5O10_22940) | 9912..10457 | - | 546 | WP_038976855.1 | DNA distortion polypeptide 1 | - |
| K5O10_RS22945 (K5O10_22945) | 10784..11056 | - | 273 | WP_000160399.1 | hypothetical protein | - |
| K5O10_RS22950 (K5O10_22950) | 11069..11218 | - | 150 | WP_000003880.1 | hypothetical protein | - |
| K5O10_RS22955 (K5O10_22955) | 11505..11834 | - | 330 | WP_000866648.1 | hypothetical protein | - |
| K5O10_RS22960 (K5O10_22960) | 11927..12142 | - | 216 | WP_001180116.1 | hypothetical protein | - |
| K5O10_RS22965 (K5O10_22965) | 12132..12377 | - | 246 | WP_000356546.1 | hypothetical protein | - |
| K5O10_RS22970 (K5O10_22970) | 12422..12745 | - | 324 | WP_001181903.1 | hypothetical protein | - |
| K5O10_RS22975 (K5O10_22975) | 12891..13172 | - | 282 | WP_000220560.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| K5O10_RS22980 (K5O10_22980) | 13162..13413 | - | 252 | WP_000121743.1 | plasmid stabilization protein | Antitoxin |
| K5O10_RS22985 (K5O10_22985) | 14669..15505 | + | 837 | WP_001050931.1 | RepB family plasmid replication initiator protein | - |
| K5O10_RS22990 (K5O10_22990) | 15545..15991 | + | 447 | WP_001074386.1 | hypothetical protein | - |
| K5O10_RS22995 (K5O10_22995) | 16128..16481 | + | 354 | WP_000876434.1 | DNA distortion polypeptide 3 | - |
| K5O10_RS23000 (K5O10_23000) | 16541..17509 | - | 969 | WP_077249520.1 | IS5-like element IS903B family transposase | - |
| K5O10_RS23005 (K5O10_23005) | 17511..17804 | - | 294 | WP_015060510.1 | hypothetical protein | - |
| K5O10_RS23010 (K5O10_23010) | 17820..18050 | - | 231 | WP_000051064.1 | plasmid partition protein ParG | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | tet(A) / floR / blaTEM-176 / aph(3')-Ia / dfrA14 / qnrS1 | - | 1..47219 | 47219 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 94 a.a. Molecular weight: 11005.83 Da Isoelectric Point: 10.5938
>T213471 WP_000220560.1 NZ_CP081814:c13172-12891 [Shigella flexneri]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR
Download Length: 282 bp
>T213471 NZ_CP109601:c3711098-3710700 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA
Antitoxin
Download Length: 84 a.a. Molecular weight: 9194.43 Da Isoelectric Point: 4.1683
>AT213471 WP_000121743.1 NZ_CP081814:c13413-13162 [Shigella flexneri]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL
Download Length: 252 bp
>AT213471 NZ_CP109601:c3711394-3711101 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | G3CAN5 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A3S5I301 |