Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4305376..4305873 Replicon chromosome
Accession NZ_CP081813
Organism Shigella flexneri strain WW1

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag K5O10_RS20690 Protein ID WP_001173343.1
Coordinates 4305376..4305549 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag K5O10_RS20695 Protein ID WP_000398619.1
Coordinates 4305577..4305873 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O10_RS20680 (4302046) 4302046..4303503 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
K5O10_RS20685 (4303740) 4303740..4305257 + 1518 WP_001295074.1 lysine--tRNA ligase -
K5O10_RS20690 (4305376) 4305376..4305549 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
K5O10_RS20695 (4305577) 4305577..4305873 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
K5O10_RS20700 (4306100) 4306100..4306372 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
K5O10_RS20705 (4306384) 4306384..4306614 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
K5O10_RS20710 (4306795) 4306795..4308426 + 1632 WP_001216460.1 sensor histidine kinase -
K5O10_RS20715 (4308423) 4308423..4309142 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T213469 WP_001173343.1 NZ_CP081813:c4305549-4305376 [Shigella flexneri]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T213469 NZ_CP109601:2639097-2639204 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT213469 WP_000398619.1 NZ_CP081813:c4305873-4305577 [Shigella flexneri]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT213469 NZ_CP109601:c2639050-2638984 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References