Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3483515..3484133 Replicon chromosome
Accession NZ_CP081813
Organism Shigella flexneri strain WW1

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag K5O10_RS16770 Protein ID WP_001291435.1
Coordinates 3483915..3484133 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag K5O10_RS16765 Protein ID WP_000344800.1
Coordinates 3483515..3483889 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O10_RS16755 (3478604) 3478604..3479797 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
K5O10_RS16760 (3479820) 3479820..3482969 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
K5O10_RS16765 (3483515) 3483515..3483889 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
K5O10_RS16770 (3483915) 3483915..3484133 + 219 WP_001291435.1 HHA domain-containing protein Toxin
K5O10_RS16775 (3484304) 3484304..3484855 + 552 WP_000102564.1 maltose O-acetyltransferase -
K5O10_RS16780 (3484971) 3484971..3485441 + 471 WP_000136192.1 YlaC family protein -
K5O10_RS16785 (3485605) 3485605..3487155 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
K5O10_RS16790 (3487197) 3487197..3487550 - 354 WP_000878141.1 DUF1428 family protein -
K5O10_RS16800 (3487929) 3487929..3488240 + 312 WP_000409911.1 MGMT family protein -
K5O10_RS16805 (3488271) 3488271..3488843 - 573 WP_000779826.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T213465 WP_001291435.1 NZ_CP081813:3483915-3484133 [Shigella flexneri]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T213465 NZ_CP109601:2286842-2287129 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT213465 WP_000344800.1 NZ_CP081813:3483515-3483889 [Shigella flexneri]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT213465 NZ_CP109601:2286603-2286842 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References