Detailed information of TA system TA213464

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2435558..2436196
• Replicon: chromosome
• Accession: NZ_CP081813
• Organism: Shigella flexneri strain WW1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: K5O10_RS11715
• Protein ID: WP_000813794.1
• Coordinates: 2436020..2436196 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: K5O10_RS11710
• Protein ID: WP_001270286.1
• Coordinates: 2435558..2435974 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
K5O10_RS11690 (2430710)	2430710..2431651	-	942	WP_001251315.1	ABC transporter permease	-
K5O10_RS11695 (2431652)	2431652..2432665	-	1014	WP_000220396.1	ABC transporter ATP-binding protein	-
K5O10_RS11700 (2432683)	2432683..2433828	-	1146	WP_000047456.1	ABC transporter substrate-binding protein	-
K5O10_RS11705 (2434073)	2434073..2435479	-	1407	WP_000760629.1	PLP-dependent aminotransferase family protein	-
K5O10_RS11710 (2435558)	2435558..2435974	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
K5O10_RS11715 (2436020)	2436020..2436196	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
K5O10_RS11720 (2436418)	2436418..2436648	+	231	WP_000494244.1	YncJ family protein	-
K5O10_RS11725 (2436740)	2436740..2438701	-	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
K5O10_RS11730 (2438774)	2438774..2439310	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
K5O10_RS11735 (2439363)	2439363..2440577	+	1215	WP_001307190.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2440617..2441882	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T213464 WP_000813794.1 NZ_CP081813:c2436196-2436020 [Shigella flexneri]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T213464 NZ_CP109601:2003906-2004009 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT213464 WP_001270286.1 NZ_CP081813:c2435974-2435558 [Shigella flexneri]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT213464 NZ_CP109601:c2004047-2003776 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• PDB: 6HPB