Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1332214..1332839 Replicon chromosome
Accession NZ_CP081813
Organism Shigella flexneri strain WW1

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag K5O10_RS06405 Protein ID WP_000911330.1
Coordinates 1332441..1332839 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag K5O10_RS06400 Protein ID WP_000450524.1
Coordinates 1332214..1332441 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O10_RS06375 (1328017) 1328017..1328487 - 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -
K5O10_RS06380 (1328487) 1328487..1329059 - 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
K5O10_RS06385 (1329205) 1329205..1330083 + 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
K5O10_RS06390 (1330100) 1330100..1331134 + 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
K5O10_RS06395 (1331347) 1331347..1332060 + 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
K5O10_RS06400 (1332214) 1332214..1332441 + 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
K5O10_RS06405 (1332441) 1332441..1332839 + 399 WP_000911330.1 type II toxin-antitoxin system VapC family toxin Toxin
K5O10_RS06410 (1332986) 1332986..1333849 + 864 WP_001267508.1 neutral zinc metallopeptidase -
K5O10_RS06415 (1333864) 1333864..1335879 + 2016 WP_000829355.1 tRNA cytosine(34) acetyltransferase TmcA -
K5O10_RS06420 (1335953) 1335953..1336651 + 699 WP_000679823.1 esterase -
K5O10_RS06425 (1336761) 1336761..1336961 - 201 WP_000383836.1 YpfN family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14894.25 Da        Isoelectric Point: 9.2216

>T213454 WP_000911330.1 NZ_CP081813:1332441-1332839 [Shigella flexneri]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T213454 NZ_CP109601:c46985-46608 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGACAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT213454 WP_000450524.1 NZ_CP081813:1332214-1332441 [Shigella flexneri]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT213454 NZ_CP109601:c47443-47075 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References