Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 1022579..1023162 Replicon chromosome
Accession NZ_CP081813
Organism Shigella flexneri strain WW1

Toxin (Protein)


Gene name mazF Uniprot ID U9XFN8
Locus tag K5O10_RS04905 Protein ID WP_000254745.1
Coordinates 1022827..1023162 (+) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag K5O10_RS04900 Protein ID WP_000581937.1
Coordinates 1022579..1022827 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O10_RS04890 (1018918) 1018918..1020219 + 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -
K5O10_RS04895 (1020267) 1020267..1022501 + 2235 WP_000226815.1 GTP pyrophosphokinase -
K5O10_RS04900 (1022579) 1022579..1022827 + 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
K5O10_RS04905 (1022827) 1022827..1023162 + 336 WP_000254745.1 endoribonuclease MazF Toxin
K5O10_RS04910 (1023233) 1023233..1024024 + 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
K5O10_RS04915 (1024252) 1024252..1025889 + 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
K5O10_RS04920 (1025977) 1025977..1027275 + 1299 WP_000036723.1 phosphopyruvate hydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12158.08 Da        Isoelectric Point: 8.4777

>T213453 WP_000254745.1 NZ_CP081813:1022827-1023162 [Shigella flexneri]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T213453 NZ_CP107728:1466710-1467099 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAATTATGGTCGCTTGCTTATAAGTCACAGAACACCGTT
TCTCGCGTCAAAATTTTAAAAATAATTGATTGTCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCTATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT213453 WP_000581937.1 NZ_CP081813:1022579-1022827 [Shigella flexneri]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT213453 NZ_CP107728:1466446-1466703 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ACCCTGATTTACTAGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYM3

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References