Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 849218..849872 Replicon chromosome
Accession NZ_CP081813
Organism Shigella flexneri strain WW1

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag K5O10_RS04135 Protein ID WP_000244772.1
Coordinates 849465..849872 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag K5O10_RS04130 Protein ID WP_000354046.1
Coordinates 849218..849484 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5O10_RS04105 (844506) 844506..845249 + 744 WP_000951964.1 SDR family oxidoreductase -
K5O10_RS04110 (845306) 845306..846739 - 1434 WP_001307385.1 6-phospho-beta-glucosidase BglA -
K5O10_RS04115 (846784) 846784..847095 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
K5O10_RS04120 (847259) 847259..847918 + 660 WP_000250274.1 hemolysin III family protein -
K5O10_RS04125 (847995) 847995..848975 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
K5O10_RS04130 (849218) 849218..849484 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
K5O10_RS04135 (849465) 849465..849872 + 408 WP_000244772.1 protein YgfX Toxin
K5O10_RS04140 (849912) 849912..850433 - 522 WP_001055874.1 flavodoxin FldB -
K5O10_RS04145 (850545) 850545..851441 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
K5O10_RS04150 (851466) 851466..852176 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
K5O10_RS04155 (852182) 852182..853915 + 1734 WP_000813220.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T213452 WP_000244772.1 NZ_CP081813:849465-849872 [Shigella flexneri]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T213452 NZ_CP107723:c32193-31888 [Escherichia coli]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT213452 WP_000354046.1 NZ_CP081813:849218-849484 [Shigella flexneri]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT213452 NZ_CP107723:c32413-32195 [Escherichia coli]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGAATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References