Detailed information of TA system TA213450

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataT-KacA/DUF1778(antitoxin)
• Location: 44039..44837
• Replicon: plasmid unnamed1
• Accession: NZ_CP081792
• Organism: Escherichia coli strain CT01

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: J6O14_RS24560
• Protein ID: WP_223571418.1
• Coordinates: 44039..44560 (-)
• Length: 174 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: A0A0V9R6Q7
• Locus tag: J6O14_RS24565
• Protein ID: WP_001351987.1
• Coordinates: 44568..44837 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O14_RS24530 (J6O14_24425)	40712..41239	+	528	WP_223571416.1	tail fiber assembly protein	-
J6O14_RS24535 (J6O14_24430)	41402..41656	+	255	WP_000120169.1	hypothetical protein	-
J6O14_RS24540	41698..42069	+	372	WP_223571417.1	hypothetical protein	-
J6O14_RS24545 (J6O14_24440)	42588..42911	+	324	WP_000064175.1	hypothetical protein	-
J6O14_RS24550 (J6O14_24445)	42925..43617	+	693	WP_096179506.1	hypothetical protein	-
J6O14_RS24555 (J6O14_24450)	43619..43870	+	252	WP_000901559.1	hypothetical protein	-
J6O14_RS24560 (J6O14_24455)	44039..44560	-	522	WP_223571418.1	GNAT family N-acetyltransferase	Toxin
J6O14_RS24565 (J6O14_24460)	44568..44837	-	270	WP_001351987.1	DUF1778 domain-containing protein	Antitoxin
J6O14_RS24570 (J6O14_24465)	45156..45824	+	669	WP_000161228.1	division plane positioning ATPase MipZ	-
J6O14_RS24575 (J6O14_24470)	45830..46195	+	366	WP_223571420.1	hypothetical protein	-
J6O14_RS24580 (J6O14_24475)	46242..47404	+	1163	WP_085947771.1	IS3-like element IS3 family transposase	-
J6O14_RS24585 (J6O14_24480)	47483..48160	-	678	WP_223571401.1	hypothetical protein	-
J6O14_RS24590 (J6O14_24485)	48533..49273	+	741	WP_223571402.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..94799	94799

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (56-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 174 a.a.
• Molecular weight: 19350.07 Da
• Isoelectric Point: 8.6369

>T213450 WP_223571418.1 NZ_CP081792:c44560-44039 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERSKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSVLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Nucleotide

• Length: 522 bp

>T213450 NZ_CP107722:62555-62866 [Escherichia coli]
ATGCACGTTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10082.54 Da
• Isoelectric Point: 7.0155

>AT213450 WP_001351987.1 NZ_CP081792:c44837-44568 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Nucleotide

• Length: 270 bp

>AT213450 NZ_CP107722:62863-63282 [Escherichia coli]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGAACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0V9R6Q7