Detailed information of TA system TA213446

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3763661..3764340
• Replicon: chromosome
• Accession: NZ_CP081791
• Organism: Escherichia coli strain CT01

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: J6O14_RS18700
• Protein ID: WP_000854680.1
• Coordinates: 3763999..3764340 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EWR7
• Locus tag: J6O14_RS18695
• Protein ID: WP_000070396.1
• Coordinates: 3763661..3763978 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O14_RS18650 (3759099)	3759099..3759920	+	822	WP_000197387.1	DUF932 domain-containing protein	-
J6O14_RS18655 (3760137)	3760137..3760838	+	702	Protein_3649	WYL domain-containing protein	-
J6O14_RS18660 (3760879)	3760879..3761115	+	237	WP_001144031.1	protein YpjK	-
J6O14_RS18665 (3761115)	3761115..3761558	+	444	WP_000649865.1	hypothetical protein	-
J6O14_RS18670 (3761581)	3761581..3762048	+	468	WP_001385283.1	protein YkfB	-
J6O14_RS18675 (3762125)	3762125..3762364	+	240	WP_000194654.1	DUF905 family protein	-
J6O14_RS18680 (3762462)	3762462..3762920	+	459	Protein_3654	antirestriction protein	-
J6O14_RS18685 (3762936)	3762936..3763412	+	477	WP_000811693.1	RadC family protein	-
J6O14_RS18690 (3763421)	3763421..3763642	+	222	WP_000691994.1	DUF987 domain-containing protein	-
J6O14_RS18695 (3763661)	3763661..3763978	+	318	WP_000070396.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
J6O14_RS18700 (3763999)	3763999..3764340	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
J6O14_RS18705 (3764583)	3764583..3765280	+	698	WP_223566534.1	IS1-like element IS1A family transposase	-
J6O14_RS18710 (3765788)	3765788..3766771	-	984	WP_223571321.1	anti-phage protein KwaB	-
J6O14_RS18715 (3766781)	3766781..3767383	-	603	WP_023565260.1	anti-phage protein KwaA	-
J6O14_RS18720 (3767990)	3767990..3768262	-	273	WP_000783295.1	ogr/Delta-like zinc finger family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3759099..3836425	77326

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T213446 WP_000854680.1 NZ_CP081791:3763999-3764340 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T213446 NZ_CP107720:c4316764-4316477 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11982.62 Da
• Isoelectric Point: 7.0270

>AT213446 WP_000070396.1 NZ_CP081791:3763661-3763978 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT213446 NZ_CP107720:c4316490-4316188 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Toxin

• AlphaFold DB: S1FHS4

Antitoxin

• AlphaFold DB: S1EWR7