Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2600085..2600723 Replicon chromosome
Accession NZ_CP081791
Organism Escherichia coli strain CT01

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag J6O14_RS12930 Protein ID WP_000813794.1
Coordinates 2600547..2600723 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag J6O14_RS12925 Protein ID WP_001270286.1
Coordinates 2600085..2600501 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O14_RS12905 (2595237) 2595237..2596178 - 942 WP_001251313.1 ABC transporter permease -
J6O14_RS12910 (2596179) 2596179..2597192 - 1014 WP_057108537.1 ABC transporter ATP-binding protein -
J6O14_RS12915 (2597210) 2597210..2598355 - 1146 Protein_2531 ABC transporter substrate-binding protein -
J6O14_RS12920 (2598600) 2598600..2600006 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
J6O14_RS12925 (2600085) 2600085..2600501 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
J6O14_RS12930 (2600547) 2600547..2600723 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
J6O14_RS12935 (2600945) 2600945..2601175 + 231 WP_000494244.1 YncJ family protein -
J6O14_RS12940 (2601267) 2601267..2603228 - 1962 WP_001488038.1 23S rRNA 5-hydroxycytidine C2501 synthase -
J6O14_RS12945 (2603301) 2603301..2603837 - 537 WP_000429169.1 DNA-binding transcriptional regulator SutR -
J6O14_RS12950 (2603929) 2603929..2605104 + 1176 WP_057108539.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T213444 WP_000813794.1 NZ_CP081791:c2600723-2600547 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T213444 NZ_CP107720:4022152-4022529 [Escherichia coli]
ATGCAAACCCAACCACTATCCTCAACGCAGGAGGCTACATCACGCCCGTCACCGGTGGAGATCTGGCAACGGCTTCTGAG
CCACCTGTTGGATCGTCACTACGGTCTGACGCTTAACGACACGCCATTTGGCAACGACGGTGTGATTCAGGAGCATATCG
ACGCCGGTATATCACTGTGCGACGCCGTGAATTTTATTGTGGAGAAGTACGATCTGGTGCGCATCGATCGTCACGGTTTC
AGTACAGAAACACAGTTGCCGCGTCTCACCAGTATCGATATTCTCCGCGCCCGCAAAACCACCGGGTTGATGACTCGCAA
CGATTACAGAACGGTGACCGACATCACCACCGGTAAATACCGTGGGGGGCATCGATGA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT213444 WP_001270286.1 NZ_CP081791:c2600501-2600085 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT213444 NZ_CP107720:4021742-4022101 [Escherichia coli]
TTGTCGAATAAAATACCGACAGTAAACCACGATATCACTGAACCCTGGTGGGGGCTTAAACGCAGTATCACGCCCTGTTT
TGGCGCACGGCTGGTGCAGTCGGGCAACTGTCTGCATTATCTCGCTGACCGGGCCAGCATCACCGGACAGTTCAGCGACG
GGGATTTACGGCATCTCGACCAGGCATTTCCACTGCTGCTCAAACAGCTTGAACTGATGCTCACCAGCGGTGAACTCACT
CCCCGCCATCAGCACTGTGTCACGCTCTATGCAAAAGGGCTGACCTGCGAGGCCGACTCCCTCGGCTCGCATGGTTACGT
ATACCTGGCGATTTATCCGACACCAGCGGCAACCGCGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References