Detailed information of TA system TA213432

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 1086868..1087451
• Replicon: chromosome
• Accession: NZ_CP081791
• Organism: Escherichia coli strain CT01

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: J6O14_RS05435
• Protein ID: WP_000254739.1
• Coordinates: 1087116..1087451 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: S1P3W5
• Locus tag: J6O14_RS05430
• Protein ID: WP_000581937.1
• Coordinates: 1086868..1087116 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O14_RS05420 (1083207)	1083207..1084508	+	1302	WP_000046826.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
J6O14_RS05425 (1084556)	1084556..1086790	+	2235	WP_000226803.1	GTP diphosphokinase	-
J6O14_RS05430 (1086868)	1086868..1087116	+	249	WP_000581937.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
J6O14_RS05435 (1087116)	1087116..1087451	+	336	WP_000254739.1	endoribonuclease MazF	Toxin
J6O14_RS05440 (1087522)	1087522..1088313	+	792	WP_057107940.1	nucleoside triphosphate pyrophosphohydrolase	-
J6O14_RS05445 (1088541)	1088541..1090178	+	1638	WP_000210878.1	CTP synthase (glutamine hydrolyzing)	-
J6O14_RS05450 (1090266)	1090266..1091564	+	1299	WP_000036723.1	phosphopyruvate hydratase	-
J6O14_RS05455 (1091708)	1091708..1091901	+	194	Protein_1069	transposase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12126.09 Da
• Isoelectric Point: 8.2618

>T213432 WP_000254739.1 NZ_CP081791:1087116-1087451 [Escherichia coli]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVVPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T213432 NZ_CP107720:1173996-1174307 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9355.74 Da
• Isoelectric Point: 4.4665

>AT213432 WP_000581937.1 NZ_CP081791:1086868-1087116 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT213432 NZ_CP107720:1174304-1174723 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LMB4