Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 715795..716582 Replicon chromosome
Accession NZ_CP081791
Organism Escherichia coli strain CT01

Toxin (Protein)


Gene name yeeV Uniprot ID A0A6L5EGB6
Locus tag J6O14_RS03545 Protein ID WP_032669201.1
Coordinates 716205..716582 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A6L5EE19
Locus tag J6O14_RS03540 Protein ID WP_023567998.1
Coordinates 715795..716154 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O14_RS03495 (711055) 711055..711768 + 714 WP_032669194.1 WYL domain-containing protein -
J6O14_RS03500 (711765) 711765..712400 + 636 WP_020837117.1 hypothetical protein -
J6O14_RS03505 (712439) 712439..712894 + 456 WP_000754598.1 hypothetical protein -
J6O14_RS03510 (712891) 712891..713340 + 450 WP_032669197.1 IrmA family protein -
J6O14_RS03515 (713412) 713412..713643 + 232 Protein_689 DUF905 domain-containing protein -
J6O14_RS03520 (713742) 713742..714564 + 823 Protein_690 DUF945 domain-containing protein -
J6O14_RS03525 (714595) 714595..715038 + 444 WP_032669199.1 antirestriction protein -
J6O14_RS03530 (715050) 715050..715529 + 480 WP_032669200.1 DNA repair protein RadC -
J6O14_RS03535 (715550) 715550..715771 + 222 WP_023567997.1 DUF987 domain-containing protein -
J6O14_RS03540 (715795) 715795..716154 + 360 WP_023567998.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
J6O14_RS03545 (716205) 716205..716582 + 378 WP_032669201.1 TA system toxin CbtA family protein Toxin
J6O14_RS03550 (716579) 716579..717070 + 492 WP_032672023.1 DUF5983 family protein -
J6O14_RS03555 (717100) 717100..717303 + 204 WP_032669203.1 DUF957 domain-containing protein -
J6O14_RS03560 (717367) 717367..718215 + 849 WP_032669204.1 DUF4942 domain-containing protein -
J6O14_RS03570 (718518) 718518..719024 + 507 WP_000228937.1 G/U mismatch-specific DNA glycosylase -
J6O14_RS03575 (719103) 719103..720944 - 1842 WP_000437371.1 RNA polymerase sigma factor RpoD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(15-114)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14157.93 Da        Isoelectric Point: 6.2236

>T213428 WP_032669201.1 NZ_CP081791:716205-716582 [Escherichia coli]
MQTQPLSSTQEATPRPSPVEIWQRLLSHLLDRHYGLTLNDTPFGNDGVIQEHIDAGISLCDAVNFIVEKYDLVRTDRRGF
NAETQSPLLSSIDILRARKATGLMTRNDYRTVTDITTGKYREVQP

Download         Length: 378 bp

>T213428 NZ_CP107720:702514-702828 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13192.04 Da        Isoelectric Point: 6.3122

>AT213428 WP_023567998.1 NZ_CP081791:715795-716154 [Escherichia coli]
MSNEIPTVNHDITEPWWGLKRSITPCFGARLVQAGNRLHYLADRANITGQFSDADLRYLDQAFPLLLKQLELMLTSGELN
PCHQHCVTLYAKGLTCEADSLGSHGYVYLAIYPTPAATA

Download         Length: 360 bp

>AT213428 NZ_CP107720:702825-703241 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCGGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCAAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6L5EGB6

Antitoxin

Source ID Structure
AlphaFold DB A0A6L5EE19

References