Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 82242..83045 Replicon plasmid unnamed2
Accession NZ_CP081784
Organism Escherichia coli strain 0H26

Toxin (Protein)


Gene name KacT Uniprot ID A0A8B2S6X5
Locus tag J6O44_RS26625 Protein ID WP_048213819.1
Coordinates 82515..83045 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A8B2S700
Locus tag J6O44_RS26620 Protein ID WP_048213818.1
Coordinates 82242..82511 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O44_RS26590 77855..77995 - 141 Protein_85 arsenical pump-driving ATPase -
J6O44_RS26595 (J6O44_26470) 78115..78687 - 573 WP_001556711.1 recombinase family protein -
J6O44_RS26600 (J6O44_26475) 78851..78991 + 141 WP_160185462.1 hypothetical protein -
J6O44_RS26605 (J6O44_26480) 79003..79707 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
J6O44_RS26610 (J6O44_26485) 79759..80115 + 357 Protein_89 site-specific integrase -
J6O44_RS26615 (J6O44_26490) 80438..81427 - 990 WP_191221256.1 RepB family plasmid replication initiator protein -
J6O44_RS26620 (J6O44_26495) 82242..82511 + 270 WP_048213818.1 DUF1778 domain-containing protein Antitoxin
J6O44_RS26625 (J6O44_26500) 82515..83045 + 531 WP_048213819.1 GNAT family N-acetyltransferase Toxin
J6O44_RS26630 (J6O44_26505) 83130..84134 - 1005 WP_048213820.1 hypothetical protein -
J6O44_RS26635 (J6O44_26510) 84139..84384 - 246 WP_048213821.1 hypothetical protein -
J6O44_RS26640 (J6O44_26515) 85380..85766 + 387 WP_059593784.1 hypothetical protein -
J6O44_RS26645 (J6O44_26520) 85778..86311 + 534 WP_065212827.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..86367 86367


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20322.17 Da        Isoelectric Point: 6.7544

>T213417 WP_048213819.1 NZ_CP081784:82515-83045 [Escherichia coli]
MDGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYLLVTREDKPRVMGYYTLSGSCFEKTLLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENSNSL
FYPTKSVEELFEVKDE

Download         Length: 531 bp

>T213417 NZ_CP107615:16664-17020 [Acinetobacter baumannii]
ATGTGGACAGTCATTACAACGGATCTGTTTAACGAATGGCTTGTACAGCAAGATGAATCTACACAAGAGAAAGTACTCGC
AGCGTTGGTCGTTCTACAGCAACAGGGACCAAGTTTAGGTCGTCCTTTAGTAGATACCGTCTATGACTCCAAATTTACCA
ACATGAAAGAGCTACGTGTTCAGCATCGAGGTAAACCACTGAGAGCCTTTTTCGCATTTGATCCTTTACGACAAGCTATT
GTGCTGTGTATCGGTGATAAAGGCGGTAAAAAGCGTTTTTATAAAGAGATGTTAGATATTGCCGATCAGCAATACGAACT
TCATCTTTCAACTTTAGGAGATCAATCTAATGGCTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10285.70 Da        Isoelectric Point: 5.7252

>AT213417 WP_048213818.1 NZ_CP081784:82242-82511 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAITNPPVPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT213417 NZ_CP107615:17013-17315 [Acinetobacter baumannii]
ATGGCTAAGACCCTACAAGAATTATTGGCTAGTCGCTCTCCAGAGAGTCAAGCTCGTATTCAAAAAATGGCTGATGAATT
ACTGTTGGAAACTCAGCTTCACCTTATTCGTGAAGAGTTAGAGATTTCTCAAAAAGAACTGGCTGCAACCCTTGGAATCA
AACAGCCGTCATTGTCAGCAATCGAAAATCGTGGTCATGATCTTAAAATTTCAACGATGAAAAAGTATGTTGAAGCAATG
GGCGGAAAACTTCGTATTGATGTAGAGCTTCCAACAGGAAAACACATAGGATTTAATGTCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B2S6X5

Antitoxin

Source ID Structure
AlphaFold DB A0A8B2S700

References