Detailed information of TA system TA213416

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 109236..109761
• Replicon: plasmid unnamed 1
• Accession: NZ_CP081783
• Organism: Escherichia coli strain 0H26

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: J6O44_RS25070
• Protein ID: WP_061301055.1
• Coordinates: 109236..109541 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: J6O44_RS25075
• Protein ID: WP_061301054.1
• Coordinates: 109543..109761 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O44_RS25050 (J6O44_24930)	104673..105437	-	765	WP_223584521.1	TnsA endonuclease N-terminal domain-containing protein	-
J6O44_RS25055 (J6O44_24935)	105443..106147	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
J6O44_RS25060 (J6O44_24940)	106703..108385	+	1683	WP_103216115.1	methyl-accepting chemotaxis protein	-
J6O44_RS25065 (J6O44_24945)	108664..109218	-	555	WP_061301056.1	DUF5710 domain-containing protein	-
J6O44_RS25070 (J6O44_24950)	109236..109541	-	306	WP_061301055.1	type II toxin-antitoxin system toxin CcdB	Toxin
J6O44_RS25075 (J6O44_24955)	109543..109761	-	219	WP_061301054.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
J6O44_RS25080 (J6O44_24960)	110161..110943	+	783	WP_061301053.1	SprT-like domain-containing protein	-
J6O44_RS25085 (J6O44_24965)	111062..111709	+	648	WP_012815856.1	ParA family protein	-
J6O44_RS25090 (J6O44_24970)	111750..111983	+	234	WP_011998900.1	hypothetical protein	-
J6O44_RS25095 (J6O44_24975)	112022..112366	-	345	WP_041460758.1	hypothetical protein	-
J6O44_RS25100 (J6O44_24980)	112423..112896	-	474	WP_011998898.1	thermonuclease family protein	-
J6O44_RS25105 (J6O44_24985)	112934..113683	-	750	WP_061301051.1	MobC family replication-relaxation protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..303840	303840
	-	flank	IS/Tn	-	-	105443..106147	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11409.04 Da
• Isoelectric Point: 4.5679

>T213416 WP_061301055.1 NZ_CP081783:c109541-109236 [Escherichia coli]
MQFIIYEYKRVSHYKMFVDVQSDIVDTPGRRMVIPLIESHHLSEIVNSSLFPVVRINDEDYRVMTTELSSVSAAVTGEAI
ADVSSDADAIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T213416 NZ_CP107615:c2069-1782 [Acinetobacter baumannii]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGATATACTAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8129.10 Da
• Isoelectric Point: 7.2032

>AT213416 WP_061301054.1 NZ_CP081783:c109761-109543 [Escherichia coli]
MKHRVSVTVDRENYQVLSSAGINISGLVNEVIGKEARKIKAEQWKADNRAGMEEVANFIAQHGSFAEENRNW

Nucleotide

• Length: 219 bp

>AT213416 NZ_CP107615:c1795-1481 [Acinetobacter baumannii]
ATGAGCATGGTTAGATACTCACACAAAGAGCTGAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA