Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 57122..57765 | Replicon | plasmid unnamed 1 |
| Accession | NZ_CP081783 | ||
| Organism | Escherichia coli strain 0H26 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | - |
| Locus tag | J6O44_RS24775 | Protein ID | WP_088765932.1 |
| Coordinates | 57122..57538 (-) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | D5KTK7 |
| Locus tag | J6O44_RS24780 | Protein ID | WP_001261282.1 |
| Coordinates | 57535..57765 (-) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J6O44_RS24730 (J6O44_24615) | 52525..53337 | + | 813 | WP_257606735.1 | plasmid replication initiator RepA | - |
| J6O44_RS24735 (J6O44_24620) | 53910..54152 | + | 243 | WP_000124732.1 | type II toxin-antitoxin system ParD family antitoxin | - |
| J6O44_RS24740 (J6O44_24625) | 54145..54429 | + | 285 | WP_000277490.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| J6O44_RS24745 (J6O44_24630) | 54567..54659 | - | 93 | Protein_59 | plasmid partition protein ParG | - |
| J6O44_RS24750 | 54979..55197 | - | 219 | Protein_60 | IS110 family transposase | - |
| J6O44_RS24755 (J6O44_24635) | 55198..55497 | - | 300 | Protein_61 | glycogen/starch synthase | - |
| J6O44_RS24760 (J6O44_24640) | 55497..56018 | - | 522 | Protein_62 | glucose-1-phosphate adenylyltransferase | - |
| J6O44_RS24765 (J6O44_24645) | 56081..56785 | + | 705 | WP_001067855.1 | IS6-like element IS26 family transposase | - |
| J6O44_RS24770 (J6O44_24650) | 56884..57024 | + | 141 | WP_045286941.1 | helix-turn-helix domain-containing protein | - |
| J6O44_RS24775 (J6O44_24655) | 57122..57538 | - | 417 | WP_088765932.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| J6O44_RS24780 (J6O44_24660) | 57535..57765 | - | 231 | WP_001261282.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| J6O44_RS24785 (J6O44_24665) | 57722..58183 | + | 462 | WP_223584524.1 | hypothetical protein | - |
| J6O44_RS24790 (J6O44_24670) | 58327..58677 | + | 351 | WP_000493399.1 | hypothetical protein | - |
| J6O44_RS27530 | 58721..58843 | + | 123 | WP_257606738.1 | hypothetical protein | - |
| J6O44_RS24795 (J6O44_24675) | 58837..59634 | - | 798 | WP_000544830.1 | IS21-like element IS21 family helper ATPase IstB | - |
| J6O44_RS24800 (J6O44_24680) | 59634..60806 | - | 1173 | WP_042111630.1 | IS21-like element IS21 family transposase | - |
| J6O44_RS24805 (J6O44_24685) | 60892..61071 | + | 180 | Protein_72 | hypothetical protein | - |
| J6O44_RS24810 (J6O44_24690) | 61123..61834 | - | 712 | Protein_73 | IS6 family transposase | - |
| J6O44_RS24815 (J6O44_24695) | 61903..62343 | + | 441 | Protein_74 | ISNCY family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | - | 1..303840 | 303840 | |
| - | inside | IScluster/Tn | - | - | 49807..84396 | 34589 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15164.56 Da Isoelectric Point: 6.4581
>T213415 WP_088765932.1 NZ_CP081783:c57538-57122 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR
Download Length: 417 bp
>T213415 NZ_CP107614:460548-460937 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTCAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCGCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCCATTAA
TTGATCAACCATTTGAATTTTCAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCGCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCCATTAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8756.83 Da Isoelectric Point: 4.4692
>AT213415 WP_001261282.1 NZ_CP081783:c57765-57535 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
Download Length: 231 bp
>AT213415 NZ_CP107614:460284-460541 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACTTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA
ATGTCTGAAGAAATGACTTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|