Detailed information of TA system TA213412

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeV-yafW/CbtA-CbeA
• Location: 4477342..4478135
• Replicon: chromosome
• Accession: NZ_CP081782
• Organism: Escherichia coli strain 0H26

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1EZ92
• Locus tag: J6O44_RS22015
• Protein ID: WP_000854726.1
• Coordinates: 4477758..4478135 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: S1EBQ7
• Locus tag: J6O44_RS22010
• Protein ID: WP_001548930.1
• Coordinates: 4477342..4477668 (+)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O44_RS21965 (4472829)	4472829..4473473	-	645	WP_001547746.1	TetR/AcrR family transcriptional regulator	-
J6O44_RS21975 (4474560)	4474560..4474796	+	237	WP_001144031.1	protein YpjK	-
J6O44_RS21980 (4474796)	4474796..4475239	+	444	WP_001547764.1	lipoprotein YafY	-
J6O44_RS21985 (4475262)	4475262..4475729	+	468	WP_001547765.1	protein YkfB	-
J6O44_RS21990 (4475806)	4475806..4476045	+	240	WP_000194654.1	DUF905 family protein	-
J6O44_RS21995 (4476143)	4476143..4476601	+	459	WP_001548150.1	antirestriction protein	-
J6O44_RS22000 (4476617)	4476617..4477093	+	477	WP_000811693.1	RadC family protein	-
J6O44_RS22005 (4477102)	4477102..4477323	+	222	WP_000691994.1	DUF987 domain-containing protein	-
J6O44_RS22010 (4477342)	4477342..4477668	+	327	WP_001548930.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
J6O44_RS22015 (4477758)	4477758..4478135	+	378	WP_000854726.1	TA system toxin CbtA family protein	Toxin
J6O44_RS22020 (4478132)	4478132..4478620	+	489	WP_001547769.1	DUF5983 family protein	-
J6O44_RS22025 (4478632)	4478632..4478829	+	198	WP_000839269.1	DUF957 domain-containing protein	-
J6O44_RS22030 (4478914)	4478914..4479756	+	843	WP_001547770.1	DUF4942 domain-containing protein	-
J6O44_RS22035 (4480505)	4480505..4482043	+	1539	WP_001187177.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4473748..4474125	377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-103)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14164.10 Da
• Isoelectric Point: 7.8045

>T213412 WP_000854726.1 NZ_CP081782:4477758-4478135 [Escherichia coli]
MKTLPDTHVREASRCPSPVTIWQTLLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T213412 NZ_CP107612:460556-460945 [Acinetobacter baumannii]
TTGATCAACCATTTGAATTTTCAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCACTCACAGAACACCATT
TCGCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCAGAGTGGAAAAAGCTTAAAACTTTAGAAAAACTCCATTAA

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12301.97 Da
• Isoelectric Point: 7.0273

>AT213412 WP_001548930.1 NZ_CP081782:4477342-4477668 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDETFPHFIRQMESMLTTGELSPHHAHCVTLYH
NDLTCEADTFGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 327 bp

>AT213412 NZ_CP107612:460292-460549 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACTTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTCGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA

Structures

Toxin

• AlphaFold DB: S1EZ92

Antitoxin

• AlphaFold DB: S1EBQ7