Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 1141690..1142265 Replicon chromosome
Accession NC_007613
Organism Shigella boydii Sb227

Toxin (Protein)


Gene name pemK Uniprot ID I6EL31
Locus tag SBO_RS06325 Protein ID WP_000373726.1
Coordinates 1141948..1142265 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID I6EL32
Locus tag SBO_RS06320 Protein ID WP_000288814.1
Coordinates 1141690..1141947 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SBO_RS26595 1136751..1137924 + 1174 Protein_1125 IS3 family transposase -
SBO_RS06295 1138087..1138296 + 210 WP_001044500.1 hypothetical protein -
SBO_RS06300 1138324..1138689 + 366 WP_000253098.1 hypothetical protein -
SBO_RS06305 1138702..1140306 + 1605 WP_000628841.1 hypothetical protein -
SBO_RS06310 1140677..1141138 + 462 WP_000201268.1 hypothetical protein -
SBO_RS06315 1141135..1141341 + 207 WP_000979502.1 helix-turn-helix domain-containing protein -
SBO_RS06320 1141690..1141947 + 258 WP_000288814.1 antitoxin Antitoxin
SBO_RS06325 1141948..1142265 + 318 WP_000373726.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
SBO_RS30380 1142425..1142571 - 147 WP_000285696.1 hypothetical protein -
SBO_RS06330 1143130..1143534 + 405 Protein_1134 DNA-binding protein -
SBO_RS06340 1143587..1144800 + 1214 WP_134810244.1 IS3 family transposase -
SBO_RS06345 1144968..1145495 + 528 Protein_1136 tyrosine-type recombinase/integrase -
SBO_RS06355 1145877..1146218 - 342 WP_000976476.1 YebY family protein -
SBO_RS06360 1146231..1147098 - 868 Protein_1138 copper homeostasis membrane protein CopD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1136751..1209537 72786
- inside IScluster/Tn - - 1143587..1152915 9328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-105)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11293.94 Da        Isoelectric Point: 10.2965

>T21341 WP_000373726.1 NC_007613:1141948-1142265 [Shigella boydii Sb227]
MDRGEIWLVSLDPTTGHEQSGKRPVLIVSKASFNTFTRLPVVVPVTSGGNFAREAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARL

Download         Length: 318 bp

>T21341 NC_007613:1141948-1142265 [Shigella boydii Sb227]
ATGGACAGAGGAGAAATCTGGCTCGTTTCACTGGATCCCACCACTGGCCATGAACAAAGTGGAAAACGTCCGGTACTCAT
CGTATCGAAGGCATCGTTTAACACGTTCACCCGGTTGCCTGTTGTTGTCCCTGTCACCAGCGGCGGAAACTTTGCCCGCG
AAGCCGGTTTTACCGTTTCACTGGACGGGGCGGGAACAAAAACAACGGGCGTTATTCGCTGCGACCAGCCCAGAACCATT
GATATGGGAGCTCGGAGCGGTAAGCGGCTGGAACGTATACCCGATGCTGTCGTGAATGAGGTGCTGGCTCGACTGTAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9280.76 Da        Isoelectric Point: 4.5821

>AT21341 WP_000288814.1 NC_007613:1141690-1141947 [Shigella boydii Sb227]
MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDNGCLIIEPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT21341 NC_007613:1141690-1141947 [Shigella boydii Sb227]
ATGTATACCACTCGCCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCCCCCGCAGTGCTGAAAACGCTAGGGCT
GTCGACGGACAGCGAAGTGGGCATGACCATTGATAATGGCTGCCTGATTATTGAACCTCAAAAACGTCCCCGCTATTCGC
TCGAAGAACTGTTGGCGCAGTGCGATCCGCACGCTGAAATAAGCGAAGAGGGTCGGGAATGGATTGATGCGCCTGCGGTG
GGCAAGGAGATCCTGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3T2UX62

Antitoxin

Source ID Structure
AlphaFold DB A0A085HLS8

References