Detailed information of TA system TA021339

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 120130..120905
• Replicon: plasmid pSB4_227
• Accession: NC_007608
• Organism: Shigella boydii Sb227

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q31SE5
• Locus tag: SBO_RS25590
• Protein ID: WP_000405246.1
• Coordinates: 120423..120905 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q7BEG8
• Locus tag: SBO_RS25585
• Protein ID: WP_010921625.1
• Coordinates: 120130..120432 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SBO_RS30340	115862..116050	-	189	WP_000957844.1	hypothetical protein	-
SBO_RS25565	116158..116412	-	255	Protein_141	transposase	-
SBO_RS25570	116804..118258	+	1455	WP_000701107.1	type 3 secretion system effector OspC2	-
SBO_RS25575	118397..119605	-	1209	WP_000198550.1	IS91 family transposase	-
SBO_RS25580	119586..119858	-	273	WP_000019158.1	hypothetical protein	-
SBO_RS25585	120130..120432	+	303	WP_010921625.1	DUF1778 domain-containing protein	Antitoxin
SBO_RS25590	120423..120905	+	483	WP_000405246.1	GNAT family N-acetyltransferase	Toxin
SBO_RS29040	120973..121496	+	524	Protein_147	transposase	-
SBO_RS25600	121539..121877	-	339	Protein_148	transposase	-
SBO_RS29050	121951..123119	+	1169	WP_094096507.1	IS3 family transposase	-
SBO_RS25615	123143..123835	-	693	WP_000625262.1	type III secretion system effector cysteine methyltransferase OspZ	-
SBO_RS29055	123891..124043	+	153	Protein_151	IS3 family transposase	-
SBO_RS30035	124280..124417	-	138	WP_011378983.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ospB / ospC4 / ospC4 / ospD2 / ospF / ospD1 / ipgB2 / ipaH1.4 / ipaH4.5 / ipaH7.8 / ospD3/senA / ospC1 / senB / ospE2 / ospI / ospC3 / ipaH9.8 / ospC2 / nleE / icsP/sopA	1..126697	126697
	-	inside	IScluster/Tn	-	ospC2	116065..123119	7054

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (29-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (19-94)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17748.55 Da
• Isoelectric Point: 7.0382

>T21339 WP_000405246.1 NC_007608:120423-120905 [Shigella boydii Sb227]
MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNASRTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHN
MPNPVPVVLLGRLAVDVCIQGHGFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV

Nucleotide

• Length: 483 bp

>T21339 NC_007608:120423-120905 [Shigella boydii Sb227]
ATGGAAATAAATGTCACCGCGCCAGCATTGTTGACGGATGAGCATATACTTCAGCCATTTGACTGTGGAAATGAGGTGCT
AAGTAACTGGTTACGTGGTCGGGCTATGAAAAACCAGATGCTCAATGCTTCTCGCACGTTCGTTATTTGCTTGGAGGATA
CTTTACGTGTTGTGGGATACTACTCATTAGCTACTGGTTCCGTCACTCATGCCGAGCTTGGTCGCAGTTTGCGGCATAAC
ATGCCCAATCCTGTCCCTGTTGTTCTGTTAGGGCGTCTGGCTGTTGATGTTTGTATCCAGGGGCATGGTTTCGGTAAATG
GCTACTAAGTGATGCCATTCACCGGGTTGTCAATCTGGCTGATCAGGTTGGCATTAAGGCTGTTATGGTACATGCAATTG
ATGATGACGCCAGAGCATTTTATGAGCGTTTTGGTTTTGTTCAGTCAGTTGTGGCACCTAATACCTTGTTTTATAAAGTC
TGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11689.28 Da
• Isoelectric Point: 4.9167

>AT21339 WP_010921625.1 NC_007608:120130-120432 [Shigella boydii Sb227]
MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRTDFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLE
APVQNAEGRERLMAVKPEWK

Nucleotide

• Length: 303 bp

>AT21339 NC_007608:120130-120432 [Shigella boydii Sb227]
ATGGAGGTTTTTATGTCTACAGCTGCAAGCGTCCGTAAAACACCGCGAGAACATCAGATTAACATCCGGGCAACTGATGA
GGAACGAGCCGTTATTGATTATGCTGCGAGCTTGGTCAATAAGAACCGTACCGATTTCATCATGGAACTGGCGTACCAGG
AAGCTAAAAACATTATCCTGGATCAACGTTTATTTGTGCTGGATAACGAACGTTATGATTCGTTTATTACGCAACTGGAA
GCTCCTGTGCAGAATGCAGAAGGGCGTGAACGTCTGATGGCGGTAAAGCCTGAATGGAAATAA

Structures

Toxin

• AlphaFold DB: Q31SE5

Antitoxin

• AlphaFold DB: Q7BEG8