Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 260935..261578 Replicon plasmid unnamed1
Accession NZ_CP081775
Organism Escherichia coli strain 0H24

Toxin (Protein)


Gene name vagD Uniprot ID -
Locus tag J6O11_RS25875 Protein ID WP_088765932.1
Coordinates 261162..261578 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag J6O11_RS25870 Protein ID WP_001261282.1
Coordinates 260935..261165 (+) Length 77 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O11_RS25835 (J6O11_25750) 256357..256797 - 441 Protein_283 ISNCY family transposase -
J6O11_RS25840 (J6O11_25755) 256866..257577 + 712 Protein_284 IS6 family transposase -
J6O11_RS25845 (J6O11_25760) 257629..257808 - 180 Protein_285 hypothetical protein -
J6O11_RS25850 (J6O11_25765) 257894..259066 + 1173 WP_042111630.1 IS21-like element IS21 family transposase -
J6O11_RS25855 (J6O11_25770) 259066..259863 + 798 WP_000544830.1 IS21-like element IS21 family helper ATPase IstB -
J6O11_RS27405 259857..259979 - 123 WP_257606738.1 hypothetical protein -
J6O11_RS25860 (J6O11_25775) 260023..260373 - 351 WP_000493399.1 hypothetical protein -
J6O11_RS25865 (J6O11_25780) 260517..260978 - 462 WP_223584524.1 hypothetical protein -
J6O11_RS25870 (J6O11_25785) 260935..261165 + 231 WP_001261282.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
J6O11_RS25875 (J6O11_25790) 261162..261578 + 417 WP_088765932.1 type II toxin-antitoxin system VapC family toxin Toxin
J6O11_RS25880 (J6O11_25795) 261676..261816 - 141 WP_045286941.1 helix-turn-helix domain-containing protein -
J6O11_RS25885 (J6O11_25800) 261915..262619 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
J6O11_RS25890 (J6O11_25805) 262682..263203 + 522 Protein_295 glucose-1-phosphate adenylyltransferase -
J6O11_RS25895 (J6O11_25810) 263203..263502 + 300 Protein_296 glycogen/starch synthase -
J6O11_RS25900 263503..263721 + 219 Protein_297 IS110 family transposase -
J6O11_RS25905 (J6O11_25815) 264041..264133 + 93 Protein_298 plasmid partition protein ParG -
J6O11_RS25910 (J6O11_25820) 264271..264555 - 285 WP_000277490.1 type II toxin-antitoxin system RelE/ParE family toxin -
J6O11_RS25915 (J6O11_25825) 264548..264790 - 243 WP_000124732.1 type II toxin-antitoxin system ParD family antitoxin -
J6O11_RS25920 (J6O11_25830) 265363..266175 - 813 WP_257606735.1 plasmid replication initiator RepA -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..303839 303839
- inside IScluster/Tn - - 234304..268893 34589


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-129)

Antitoxin

(7-47)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15164.56 Da        Isoelectric Point: 6.4581

>T213387 WP_088765932.1 NZ_CP081775:261162-261578 [Escherichia coli]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR

Download         Length: 417 bp

>T213387 NZ_CP107593:c2617003-2616821 [Acinetobacter baumannii]
GTGAAAAGTCTGGATTTAATCAAAATGATTGAAGCAGACGGTTGGTATGAGGTTAGGGTTTCAGGAAGTCATCATCACTT
CAAACACCCAACCAAAAAGGGATTAGTTACTATCCCGCATCCTAAAAAGGATTTACCAAGCGGAACTGTTAAAAGCATTT
TGAAGCAAGCGGGTCTAAAGTGA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT213387 WP_001261282.1 NZ_CP081775:260935-261165 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT213387 NZ_CP107593:c2616756-2616352 [Acinetobacter baumannii]
ATGTTGTATCCAATTGCTATTGAGAGAGGTACAGACACCGAGGCCTTTGGTGTCACCGTTCCAGATATTCCAGGATGTTT
TAGCGCAGGCGATACATTAGAGGAAGCTATCGAGAACGTTAAAGAGGCAATTTCTGGCCACTTAGAAATCCTTGCTGAAG
ATGGAGAAGAAATTCCATTAGCATCTGACTTAGCAAAATTTGTAGATGATCCAGATTATAAAGGCATGATCTGGGCTGTT
ACTGAGGTGGATGTTAGTCGTTATCTTGGTAAGCCTGAAAAAATCAATGTTACTTTACCAAGCCGTTTGATTCGTAAAAT
TGATGAGAATGTAGGTAAAGGTAAGAGATATACTACTCGATCGGCTTTCTTGGCTGCTGGTGCTGAAAAACTTTTACATG
CATAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References