Detailed information of TA system TA213386

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 208939..209464
• Replicon: plasmid unnamed1
• Accession: NZ_CP081775
• Organism: Escherichia coli strain 0H24

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: J6O11_RS25580
• Protein ID: WP_061301055.1
• Coordinates: 209159..209464 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: J6O11_RS25575
• Protein ID: WP_061301054.1
• Coordinates: 208939..209157 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J6O11_RS25545 (J6O11_25460)	205017..205766	+	750	WP_061301051.1	MobC family replication-relaxation protein	-
J6O11_RS25550 (J6O11_25465)	205804..206277	+	474	WP_011998898.1	thermonuclease family protein	-
J6O11_RS25555 (J6O11_25470)	206334..206678	+	345	WP_041460758.1	hypothetical protein	-
J6O11_RS25560 (J6O11_25475)	206717..206950	-	234	WP_011998900.1	hypothetical protein	-
J6O11_RS25565 (J6O11_25480)	206991..207638	-	648	WP_012815856.1	ParA family protein	-
J6O11_RS25570 (J6O11_25485)	207757..208539	-	783	WP_061301053.1	SprT-like domain-containing protein	-
J6O11_RS25575 (J6O11_25490)	208939..209157	+	219	WP_061301054.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
J6O11_RS25580 (J6O11_25495)	209159..209464	+	306	WP_061301055.1	type II toxin-antitoxin system toxin CcdB	Toxin
J6O11_RS25585 (J6O11_25500)	209482..210036	+	555	WP_061301056.1	DUF5710 domain-containing protein	-
J6O11_RS25590 (J6O11_25505)	210315..211997	-	1683	WP_103216115.1	methyl-accepting chemotaxis protein	-
J6O11_RS25595 (J6O11_25510)	212553..213257	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
J6O11_RS25600 (J6O11_25515)	213263..214027	+	765	WP_223584521.1	TnsA endonuclease N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..303839	303839
	-	flank	IS/Tn	-	-	212553..213257	704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11409.04 Da
• Isoelectric Point: 4.5679

>T213386 WP_061301055.1 NZ_CP081775:209159-209464 [Escherichia coli]
MQFIIYEYKRVSHYKMFVDVQSDIVDTPGRRMVIPLIESHHLSEIVNSSLFPVVRINDEDYRVMTTELSSVSAAVTGEAI
ADVSSDADAIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T213386 NZ_CP107593:464914-465303 [Acinetobacter baumannii]
TTGATCAACCATTCGAATTTTAAGCTGAAATATAGTCGTTTTTCGATTATATTTCAGTTTTTTATTGGCTTAAGCTTAGC
AGTTCTATTTTATCAATTAATGCCGTTACTATGGTGGTTAGTTGTAATCAGCTTACTATTTATCAGCTTTATTTTCTTTT
TAAAGCGTCCGCAGCTTGCACAAATTGCTTATTTAGATAAAAAACTATGGTCGCTTGCTTATCATTCACAGAACACCATT
TCTCGCGTCAAAATTTTAAAAATAATTGATTATCAGATTTTCATAGTGATCTATTTTGAAGGTCATAAAACACTAACTTC
TATCATTTGGTTTGATCAAATGTCATTAGCGGAGTGGAAGAAGCTTAAAACTTTAGAAAAACTCCATTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8129.10 Da
• Isoelectric Point: 7.2032

>AT213386 WP_061301054.1 NZ_CP081775:208939-209157 [Escherichia coli]
MKHRVSVTVDRENYQVLSSAGINISGLVNEVIGKEARKIKAEQWKADNRAGMEEVANFIAQHGSFAEENRNW

Nucleotide

• Length: 219 bp

>AT213386 NZ_CP107593:464650-464907 [Acinetobacter baumannii]
ATGTCTGAAGAAATGACCTTGGAAGAACGTAAAGTAATCTATCGCGCACGTCGTGGTTTAAAGGAAATTGATGTCTATTT
TGACCCTTATGTGAAAAATTATTATTTAAAGGCAGACCCGGCAGAAAAAGCGTTGTTTGCTGAGTTGGTAGAACAGGAAG
ATCCTGATTTACTTGATTGGTTTATGGAAGTATCAGAACCACCACGTACCGAATTACGTGACTTTATCTATAAATTAAAA
CACTATGTTCATGGTTAA