Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 91598..92123 Replicon plasmid pSB4_227
Accession NC_007608
Organism Shigella boydii Sb227

Toxin (Protein)


Gene name ccdB Uniprot ID Q326Z8
Locus tag SBO_RS25415 Protein ID WP_001159860.1
Coordinates 91818..92123 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q7BEK0
Locus tag SBO_RS25410 Protein ID WP_000813626.1
Coordinates 91598..91816 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
SBO_RS28990 87442..88598 + 1157 WP_094096646.1 IS3 family transposase -
SBO_RS29865 88642..88809 + 168 Protein_106 IS3 family transposase -
SBO_RS29870 88827..88958 - 132 Protein_107 IS110 family transposase -
SBO_RS25400 90266..90913 + 648 Protein_109 IS3-like element IS911 family transposase -
SBO_RS25410 91598..91816 + 219 WP_000813626.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
SBO_RS25415 91818..92123 + 306 WP_001159860.1 type II toxin-antitoxin system toxin CcdB Toxin
SBO_RS29875 92147..92254 + 108 WP_023592908.1 transposase domain-containing protein -
SBO_RS25425 92516..94153 + 1638 WP_000936800.1 T3SS effector E3 ubiquitin-protein ligase IpaH9.8 -
SBO_RS25430 94361..94630 + 270 WP_000079956.1 hypothetical protein -
SBO_RS30625 94627..94799 + 173 Protein_115 type II toxin-antitoxin system RelE/ParE family toxin -
SBO_RS25435 94863..95560 - 698 Protein_116 IS1 family transposase -
SBO_RS29005 95767..95883 + 117 WP_004996477.1 hypothetical protein -
SBO_RS25445 96247..97089 + 843 WP_000931198.1 polysaccharide deacetylase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospB / ospC4 / ospC4 / ospD2 / ospF / ospD1 / ipgB2 / ipaH1.4 / ipaH4.5 / ipaH7.8 / ospD3/senA / ospC1 / senB / ospE2 / ospI / ospC3 / ipaH9.8 / ospC2 / nleE / icsP/sopA 1..126697 126697
- inside IScluster/Tn - ospE2 / ospI / ospC3 / ipaH9.8 70356..101488 31132


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11736.59 Da        Isoelectric Point: 6.4674

>T21337 WP_001159860.1 NC_007608:91818-92123 [Shigella boydii Sb227]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T21337 NC_007608:91818-92123 [Shigella boydii Sb227]
ATGCAGTTTAAGGTTTACGCCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGACGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACTGATATGGCCAGTGTGCCGATCTTCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8333.36 Da        Isoelectric Point: 6.2838

>AT21337 WP_000813626.1 NC_007608:91598-91816 [Shigella boydii Sb227]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT21337 NC_007608:91598-91816 [Shigella boydii Sb227]
ATGAAGCAGCGTATTACTGTCACTATAGACAGTGACAGCTATCAGTTACTTAAATCCGCAAACGTGAATATCTCAGGCCT
GGTAAATACGGCCATGCAAAAAGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCTGAAAATCAGCAGGGGATGGCTG
AGATCGCCCGTTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTN3

Antitoxin

Source ID Structure
AlphaFold DB Q7BEK0

References