Detailed information of TA system TA021336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 35093..35850
• Replicon: plasmid pSB4_227
• Accession: NC_007608
• Organism: Shigella boydii Sb227

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: Q31SN7
• Locus tag: SBO_RS25065
• Protein ID: WP_000501969.1
• Coordinates: 35365..35850 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: Q31SN8
• Locus tag: SBO_RS25060
• Protein ID: WP_011114751.1
• Coordinates: 35093..35377 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SBO_RS25035	31036..32016	+	981	WP_000431558.1	ParB/RepB/Spo0J family partition protein	-
SBO_RS25040	32272..32754	+	483	Protein_41	IS110 family transposase	-
SBO_RS30305	32808..32962	-	155	Protein_42	transposase domain-containing protein	-
SBO_RS30505	33045..33803	-	759	Protein_43	group II intron reverse transcriptase/maturase	-
SBO_RS30605	33798..34420	-	623	Protein_44	transposase	-
SBO_RS25060	35093..35377	+	285	WP_011114751.1	DUF1778 domain-containing protein	Antitoxin
SBO_RS25065	35365..35850	+	486	WP_000501969.1	GNAT family N-acetyltransferase	Toxin
SBO_RS30610	35935..36309	+	375	Protein_47	group II intron reverse transcriptase/maturase	-
SBO_RS30615	36304..36855	+	552	Protein_48	IS3 family transposase	-
SBO_RS25075	36913..38040	-	1128	WP_001063816.1	IS110-like element ISSso6 family transposase	-
SBO_RS25080	38539..39327	+	789	WP_005116773.1	AraC family invasion system transcriptional regulator VirF	-
SBO_RS25085	39320..39691	-	372	Protein_51	ATP-binding protein	-
SBO_RS25090	39690..40057	+	368	Protein_52	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ospB / ospC4 / ospC4 / ospD2 / ospF / ospD1 / ipgB2 / ipaH1.4 / ipaH4.5 / ipaH7.8 / ospD3/senA / ospC1 / senB / ospE2 / ospI / ospC3 / ipaH9.8 / ospC2 / nleE / icsP/sopA	1..126697	126697
	-	inside	IScluster/Tn	-	ipaH1.4	34118..48822	14704

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (30-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (12-88)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17712.54 Da
• Isoelectric Point: 10.1083

>T21336 WP_000501969.1 NC_007608:35365-35850 [Shigella boydii Sb227]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T21336 NC_007608:35365-35850 [Shigella boydii Sb227]
ATGGGATGTGTAACTGCACCAGAACCTTTATCCTCATTTCATCAGGTCGCTGAATTTGTCAGCGGTGAAGCTGTACTTGA
TGACTGGTTAAAGCAGAAGGGGCTAAAAAATCAGGCAATCGGAGCCACCCGGACTTTTGTTGTATGCAGAAAAGGTACGC
AACAAATAGTCGGTTTTTACTCACTGGCCACTGGTAGTGTCAATCATACAGAAGCTACAGGTAACCTTCGGCGCAATATG
CCAGACCCCATTCCTGTAATTATACTTGCACGCCTGGCTGTAGATGTGTCATTTCGAGGAAAAGGGCTTGGCGCGGATTT
ACTTCATGATGCAGTTCGTCGTTGTTACAGGGTTGCCGAAAATATCGGTGTGCGTGCAATTATGGTTCATGCGCTTACGG
AAAACGCAAAACAGTTTTATATTCATCATGGTTTTAAGCCATCAAAAACTCAGGTGCAAACATTGTTTCTTAAACTCCCT
CAATAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10871.52 Da
• Isoelectric Point: 4.6812

>AT21336 WP_011114751.1 NC_007608:35093-35377 [Shigella boydii Sb227]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Nucleotide

• Length: 285 bp

>AT21336 NC_007608:35093-35377 [Shigella boydii Sb227]
ATGATATACGGAGGCTTTATGAAATCAGGTGTTCAGCTCAATCTCAGAGCAAGAGAATCACAGCGGATACTCATTGATGC
TGCTGCAGAAATTCTTCATAAGTCGCGTACAGATTTCATTCTGGAAATGGCCTGTAAAGCTGCTGAAGATGTTATTCTCG
ACCGGCGGGTGTTTAATTTTAATGACAGGCAATATGAAGAGTTCATTGAAATGCTTGATGCTCCGGTTGCAGATGATCCT
GCCATCGAAAAATTGCTGGCAAGGAAACCTCAATGGGATGTGTAA

Structures

Toxin

• AlphaFold DB: Q31SN7

Antitoxin

• AlphaFold DB: A0A4V1CUJ1