Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/ElaA-DUF1778
Location 70931..71734 Replicon plasmid unnamed3
Accession NZ_CP081769
Organism Escherichia coli strain 0H18

Toxin (Protein)


Gene name KacT Uniprot ID A0A8B2S6X5
Locus tag J6O59_RS26565 Protein ID WP_048213819.1
Coordinates 71204..71734 (+) Length 177 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A8B2S700
Locus tag J6O59_RS26560 Protein ID WP_048213818.1
Coordinates 70931..71200 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J6O59_RS26530 66544..66684 - 141 Protein_73 arsenical pump-driving ATPase -
J6O59_RS26535 (J6O59_26375) 66804..67376 - 573 WP_001556711.1 recombinase family protein -
J6O59_RS26540 (J6O59_26380) 67540..67680 + 141 WP_160185462.1 hypothetical protein -
J6O59_RS26545 (J6O59_26385) 67692..68396 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
J6O59_RS26550 (J6O59_26390) 68448..68804 + 357 Protein_77 site-specific integrase -
J6O59_RS26555 (J6O59_26395) 69127..70116 - 990 WP_191221256.1 RepB family plasmid replication initiator protein -
J6O59_RS26560 (J6O59_26400) 70931..71200 + 270 WP_048213818.1 DUF1778 domain-containing protein Antitoxin
J6O59_RS26565 (J6O59_26405) 71204..71734 + 531 WP_048213819.1 GNAT family N-acetyltransferase Toxin
J6O59_RS26570 (J6O59_26410) 71819..72823 - 1005 WP_048213820.1 hypothetical protein -
J6O59_RS26575 (J6O59_26415) 72828..73073 - 246 WP_048213821.1 hypothetical protein -
J6O59_RS26580 (J6O59_26420) 74069..74455 + 387 WP_059593784.1 hypothetical protein -
J6O59_RS26585 (J6O59_26425) 74467..75000 + 534 WP_065212827.1 hypothetical protein -
J6O59_RS26590 (J6O59_26430) 75005..75784 + 780 WP_050316692.1 site-specific integrase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..86368 86368


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(92-156)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 177 a.a.        Molecular weight: 20322.17 Da        Isoelectric Point: 6.7544

>T213359 WP_048213819.1 NZ_CP081769:71204-71734 [Escherichia coli]
MDGLRIEIFSEEVEYELSNFDCGEEYLNTFLTDHLKRQHNSKILRGYLLVTREDKPRVMGYYTLSGSCFEKTLLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENSNSL
FYPTKSVEELFEVKDE

Download         Length: 531 bp

>T213359 NZ_CP107574:609370-609702 [Pantoea dispersa]
ATGGTGTCACGTTTTGTTCCCGATGCAGGCGATCTCATCTGGCTGGATTTTGATCCCACCCTCGGCCATGAACAGGGCGG
CCATCGTCCTGCGGTTGTGCTGAGTCCCTTTGCCTATAACAACAAGGTTGGCATGTTGTTGTGCGTCCCGTGTACCACCC
AGGTTAAAGGCTATCCTTTTGAAGTTTCCCTTACCGGTAGCCGTGATAGTGTGGCGCTGGCCGATCAGGTCACCTCAATC
GACTGGCGCGCCAGAAAAGTGGTGAAAAAAAGCCATGTGACCGCCGACGAACTGAGTGAGATTCGCGCTAAAGCTAAAGC
GCTGATTGGCTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10285.70 Da        Isoelectric Point: 5.7252

>AT213359 WP_048213818.1 NZ_CP081769:70931-71200 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASERAAEVIDQHRRLLLNEESWNLVMDAITNPPVPNDRLKR
AANRLRELE

Download         Length: 270 bp

>AT213359 NZ_CP107574:609125-609370 [Pantoea dispersa]
ATGACGACCGTCACCTTAAAGAAATGGGGCAACAGTCCCTCGATTCGCATTCCGTCCAGCGTGATGCAATCCGCGTCGCT
TAGCGTGGACGATAAACTGGAACTCAGCGTTGAAGATGGCAAGCTGATCCTTGTGCCGCTTAGGGAAAAGGTGTATTCAC
TGGACGCTCTGCTGGACGAGATCACCGACGATAACGTCCATGATAAAATTGATTTTGGACCTTCAGTGGGCAAGGAAAGA
ATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A8B2S6X5

Antitoxin

Source ID Structure
AlphaFold DB A0A8B2S700

References